BLOOD VESSEL STAINING KIT

Species Reactivity	Key Applications	Detection Methods
H	IHC, IH(P)	Chromogenic

Species Reactivity

Key Applications

Detection Methods

IHC, IH(P)

Chromogenic

Description
Catalogue Number	ECM590
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Blood Vessel Staining Kit
Overview	Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date. ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31. Background: Angiogenesis and tumor-associated neovascularization are complex processes by which new blood vessels are formed from pre-existing vasculature. Several studies implicate tumor-associated blood vessel formation as a central part in the process of growth, invasion and metastasis of malignancies (Rak et al., 1995; Jahroudi & Greenberger, 1995; Saphir, 1997). The most widely used endothelial cell markers for studying angiogenesis/neovascularization are von Willebrand Factor (Factor VIII Related Antigen) and CD31 (PECAM-1). von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. CHEMICON's von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors. It is important to note that not all endothelial cells synthesize von Willebrand factor, therefore a small percentage of these vascular-rich tumors might not stain with this antibody. CD31 or platelet endothelial adhesion molecule (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin super family of cell surface receptors. It is strongly expressed by all endothelial cells and weakly by several types of leucocytes (Parums et al., 1990). According to several studies, staining of blood vessels with CD31 antibodies has shown to be suitable for the identification of angiogenesis in several types of malignancies such as lung (Giatromanolaki et al., 1996), breast (Horak et al., 1992) and colorectal cancer (Takebayashi et al., 1996). CHEMICON's monoclonal antibody to CD31 is very specific for the staining of vascular endothelial cells in normal and cancerous tissue. CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This immunoperoxidase kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues
Materials Required but Not Delivered	1. Tissue Sections/Slides: Paraffin-embedded or frozen sections can be used with this kit. 2. 100% Ethanol. 3. Xylene. 4. Hematoxylin: If counterstaining if required. 5. Solutions used for epitope/antigen retrieval: For example, 10 mM Sodium Citrate (pH 6.0) solution. 6. Hydrogen Peroxide: Needed if quenching of endogenous peroxidase activity is required. 7. Mounting Fluid: Aqueous or Xylene-based. 8. Coverslips. 9. Microscope.

Description

Catalogue Number

ECM590

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Blood Vessel Staining Kit

Overview

Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date. ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.

Background:

Angiogenesis and tumor-associated neovascularization are complex processes by which new blood vessels are formed from pre-existing vasculature. Several studies implicate tumor-associated blood vessel formation as a central part in the process of growth, invasion and metastasis of malignancies (Rak et al., 1995; Jahroudi & Greenberger, 1995; Saphir, 1997).

The most widely used endothelial cell markers for studying angiogenesis/neovascularization are von Willebrand Factor (Factor VIII Related Antigen) and CD31 (PECAM-1). von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. CHEMICON's von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors. It is important to note that not all endothelial cells synthesize von Willebrand factor, therefore a small percentage of these vascular-rich tumors might not stain with this antibody. CD31 or platelet endothelial adhesion molecule (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin super family of cell surface receptors. It is strongly expressed by all endothelial cells and weakly by several types of leucocytes (Parums et al., 1990). According to several studies, staining of blood vessels with CD31 antibodies has shown to be suitable for the identification of angiogenesis in several types of malignancies such as lung (Giatromanolaki et al., 1996), breast (Horak et al., 1992) and colorectal cancer (Takebayashi et al., 1996). CHEMICON's monoclonal antibody to CD31 is very specific for the staining of vascular endothelial cells in normal and cancerous tissue.

CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This immunoperoxidase kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues

Materials Required but Not Delivered

1. Tissue Sections/Slides: Paraffin-embedded or frozen sections can be used with this kit.

2. 100% Ethanol.

3. Xylene.

4. Hematoxylin: If counterstaining if required.

5. Solutions used for epitope/antigen retrieval: For example, 10 mM Sodium Citrate (pH 6.0) solution.

6. Hydrogen Peroxide: Needed if quenching of endogenous peroxidase activity is required.

7. Mounting Fluid: Aqueous or Xylene-based.

8. Coverslips.

9. Microscope.

Product Information
Components	Rabbit anti-vWF Polyclonal Antibody (Part No. 21540): One vial containing 100 μg at 1 mg/mL. Mouse anti-CD31 Monoclonal Antibody (Part No. 90214): One vial containing 100 μL at 15 mg/mL (total protein including IgG and Carrier protein). Goat anti-Rabbit Secondary Antibody (Part No. 21537): One 15 mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein. Goat anti-Mouse Secondary Antibody (Part No. 21538): One 15 mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein. Blocking Reagent (Part No. 20783): One 15 mL ready-to-use solution containing normal goat serum in PBS with a carrier protein. Streptavidin-HRP (Part No. 90215): One 15 ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS). DAB Chromogen A (Part No. 90216): One 5 mL bottle of 3, 3' Diaminobenzidine diluted in TBS. DAB Chromogen B (Part No: 90217): Two 12 mL bottles of hydrogen peroxide diluted in distilled water. 20X Rinse Buffer (Part No. 90218): One 275 mL bottle of 20X TBS.
Detection method	Chromogenic

Product Information

Components

Rabbit anti-vWF Polyclonal Antibody (Part No. 21540): One vial containing 100 μg at 1 mg/mL.
Mouse anti-CD31 Monoclonal Antibody (Part No. 90214): One vial containing 100 μL at 15 mg/mL (total protein including IgG and Carrier protein).
Goat anti-Rabbit Secondary Antibody (Part No. 21537): One 15 mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein.
Goat anti-Mouse Secondary Antibody (Part No. 21538): One 15 mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein.
Blocking Reagent (Part No. 20783): One 15 mL ready-to-use solution containing normal goat serum in PBS with a carrier protein.
Streptavidin-HRP (Part No. 90215): One 15 ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS).
DAB Chromogen A (Part No. 90216): One 5 mL bottle of 3, 3' Diaminobenzidine diluted in TBS.
DAB Chromogen B (Part No: 90217): Two 12 mL bottles of hydrogen peroxide diluted in distilled water.
20X Rinse Buffer (Part No. 90218): One 275 mL bottle of 20X TBS.

Detection method

Chromogenic

Applications
Application	Reagents & materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections & when stored at 2-8ºC is stable up to the expiration date.
Key Applications	Immunohistochemistry Immunohistochemistry (Paraffin)
Application Notes	ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.

Applications

Application

Reagents & materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections & when stored at 2-8ºC is stable up to the expiration date.

Key Applications

Immunohistochemistry
Immunohistochemistry (Paraffin)

Application Notes

ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.

Biological Information
Species Reactivity	Human

Biological Information

Species Reactivity

Human

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight.

Storage and Shipping Information

Storage Conditions

Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight.

Packaging Information
Material Size	1 kit
Material Package	sufficient for 100 sections

Packaging Information

Material Size

1 kit

Material Package

sufficient for 100 sections

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

BLOOD VESSEL STAINING KIT

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