CpG Wiz® Prader-Willi/ Angelman Amplification Kit

Description
Catalogue Number	S7806
Brand Family	Chemicon®
Trade Name	CpG Wiz Chemicon
Description	CpG WIZ® Prader-Willi/Angelman -Methylation specific PCR assay
Overview	A differentially methylated site exists in the CpG islands of SNRPN (small nuclear ribonucleoprotein-associated polypeptide N), a candidate gene for Prader-Willi/Angelman Syndrome. Normally, this site is methylated and inactive on the maternal homolog and unmethylated and transcriptionally active on the paternal homolog. In research samples from Prader-Willi individuals, only the methylated allele is present, while samples from Angelman Syndrome (AS) individuals contain only the unmethlyated allele. The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also included. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

Description

Catalogue Number

S7806

Brand Family

Chemicon®

Trade Name

CpG Wiz
Chemicon

Description

CpG WIZ® Prader-Willi/Angelman -Methylation specific PCR assay

Overview

A differentially methylated site exists in the CpG islands of SNRPN (small nuclear ribonucleoprotein-associated polypeptide N), a candidate gene for Prader-Willi/Angelman Syndrome. Normally, this site is methylated and inactive on the maternal homolog and unmethylated and transcriptionally active on the paternal homolog. In research samples from Prader-Willi individuals, only the methylated allele is present, while samples from Angelman Syndrome (AS) individuals contain only the unmethlyated allele. The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also included.

Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

Applications
Application	The CpG WIZ Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation-specific PCR (MSP).
Key Applications	PCR
Application Notes	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG Wiz® Prader-Willi/Angelman Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

Applications

Application

The CpG WIZ Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation-specific PCR (MSP).

Key Applications

Application Notes

Principles of the Technique

Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG Wiz® Prader-Willi/Angelman Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

Biological Information
Species Reactivity	Human
Entrez Gene Number	NM_144599.3
Entrez Gene Summary	This gene encodes a potential transmembrane protein which may function as a receptor or transporter molecule. This protein may play a role in nervous system development and maintenance. Alternative splice variants have been discribed, but their biological nature has not been determined. Mutations in this gene have been associated with autosomal dominant spastic paraplegia 6.
Gene Symbol	NIPA1 SPG6 FSP3 MGC35570 MGC102724
UniProt Number	Q7RTP0
UniProt Summary	SIZE: 329 amino acids; 34562 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential). TISSUE SPECIFICITY: Widely expressed with highest levels in neuronal tissues. DISEASE: SwissProt: Q7RTP0 # Defects in NIPA1 are the cause of spastic paraplegia type 6 (SPG6) [MIM:600363]. SPG6 is a form of autosomal dominant hereditary spastic paraplegia (AD-HSP). HSP is a group of inherited degenerative spinal cord disorders characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SIMILARITY: SwissProt: Q7RTP0 ## Belongs to the NIPA family.

Biological Information

Species Reactivity

Human

Entrez Gene Number

NM_144599.3

Entrez Gene Summary

This gene encodes a potential transmembrane protein which may function as a receptor or transporter molecule. This protein may play a role in nervous system development and maintenance. Alternative splice variants have been discribed, but their biological nature has not been determined. Mutations in this gene have been associated with autosomal dominant spastic paraplegia 6.

Gene Symbol

NIPA1
SPG6
FSP3
MGC35570
MGC102724

UniProt Number

Q7RTP0

UniProt Summary

SIZE: 329 amino acids; 34562 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Widely expressed with highest levels in neuronal tissues.
DISEASE: SwissProt: Q7RTP0 # Defects in NIPA1 are the cause of spastic paraplegia type 6 (SPG6) [MIM:600363]. SPG6 is a form of autosomal dominant hereditary spastic paraplegia (AD-HSP). HSP is a group of inherited degenerative spinal cord disorders characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
SIMILARITY: SwissProt: Q7RTP0 ## Belongs to the NIPA family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging Information
Material Size	25 assays

Packaging Information

Material Size

25 assays

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

CpG Wiz® Prader-Willi/ Angelman Amplification Kit

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