[关闭]
您好！欢迎来到炼石商城   请登录  注册    兼职小伙伴  
我的购物车
0
Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

图片仅供参考，请以实物为准
收藏

CpG Wiz® RARb2 Amplification Kit

市场价：
0.00
促销价：
¥0.00

货号：S7814

品牌：Millipore 密理博

配送至：

支付： 账期 货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次
RNeasy Mini Kit (50) 50次
3720.00
Plasmid Mini Kit I(200) 200次
Plasmid Mini Kit I(200) 200...
576.00
10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱
10ul吸头，透明，袋装，可灭菌...
85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10
QIAfilter Plasmid Maxi Kit (...
3000.00
QIAamp DNA Blood Midi Kit (20) 20次
QIAamp DNA Blood Midi Kit (2...
2680.00
QIAamp UCP Pathogen Mini Kit (50)
QIAamp UCP Pathogen Mini Kit...
4112.00
Replacement Information
Description
Catalogue Number S7814
Brand Family Chemicon®
Trade Name
  • CpG Wiz
  • Chemicon
Description CpG WIZ® RARβ2 - Methylation specific PCR assay
Overview Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). The Retinoic Acid Receptor beta2 is expressed at reduced levels in many tumor types as a result of hypermethylation of its promoter region.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
Materials Required but Not Delivered Equipment and Supplies

a. Microcentrifuge tubes for PCR amplification

b. Aerosol-resistant pipette tips

c. Thermocycler

d. Gel electrophoresis apparatus (vertical or horizontal)

e. Power Supply

f. 302 nm UV transilluminator, camera and film

Reagents

a 2.5 mM dNTP mix (2.5 mM of each nucleotide)

b. "Hot start" Taq polymerase

c. "Hot start" PCR reagents (see Sec. II. Protocols).

d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide, or suitable high resolution agarose)

e. DNA markers (size range 100-300 bp)

f. Ethidium bromide (10 mg/mL)

g. Gel-loading solution / Loading Dye

h. Bisulfite Modified DNA (CpGenome™ DNA Modification Kit, Cat. No. S7820)
References
Product Information
Components
  • The components of the CpG WIZ® RARbeta2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
  • U Primer Set7.5 μM each primer (25X) 35 μL (clear cap) 90595 -15°C to -25°C
  • M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90596 -15°C to -25°C
  • W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90597 -15°C to -25°C
  • U control DNA0.1 μg/μL 50 μL (clear cap) 90393 -15°C to -25°C
  • M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
  • W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C
  • Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
Applications
Application The components of the CpG WIZ RARbeta2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
Key Applications
  • PCR
Application Notes Principles of the Technique

Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® RARbeta2 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
Biological Information
Species Reactivity
  • Human
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
Gene Symbol
  • RXRB
  • H-2RIIBP
  • NR2B2
  • MGC1831
  • RCoR-1
  • DAUDI6
UniProt Number
UniProt Summary FUNCTION: SwissProt: P28702 # Nuclear hormone receptor. Involved in the retinoic acid response pathway. Binds 9-cis retinoic acid (9C-RA).
SIZE: 533 amino acids; 56922 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in a variety of tumor cell lines.
DOMAIN: SwissProt: P28702 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR2 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 25 assays
Transport Information
Supplemental Information
Specifications

温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！

我要咨询

咨询内容：
购物指南 免费注册 批量采购 购物流程 购物保障
配送方式 上门自提 第三方配送 炼石配送
支付方式 货到付款 账期付款
售后服务 售后政策 退款说明 售后流程 免责条款

服务热线

0771-3293894

关于我们|帮助中心|诚聘英才|炼石云实验室|联系我们|友情链接

Copyright 2014-2025 炼石云商网络科技（海南）有限公司版权所有 琼ICP备2023006525号-3