Ms X MAP-2 Alexa Fluor® 488

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Ms X MAP-2 Alexa Fluor® 488

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货号：MAB3418X

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, Ch, H, M, R	IHC	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	MAB3418X
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MAP2 Antibody, Alexa Fluor® 488 conjugated

References

Product Information
Format	AlexaFluor®488
Control	Brain, Neuronal culture, Human glioblastoma T98G cells
Presentation	Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Applications
Application	This Anti-MAP2 Antibody, Alexa Fluor 488 conjugated is validated for use in IH for the detection of MAP2.
Key Applications	Immunohistochemistry
Application Notes	Immunohistochemistry Optimal working dilutions must be determined by end user. APPLICATION NOTES: Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

Biological Information
Immunogen	Bovine brain microtubule protein.
Clone	AP20
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1). MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5). SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Isotype	IgG1
Species Reactivity	Bovine Chicken Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001039538.1 NM_002374.3 NM_031845.2 NM_031847.2
Entrez Gene Summary	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol	MAP2 MAP2B MAP-2 MAP2C DKFZp686I2148 MAP2A
Purification Method	Protein A Purfied
UniProt Number	P11137
UniProt Summary	FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.