Glial Fibrillary Acidic Protein Porcine

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Glial Fibrillary Acidic Protein Porcine

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货号：AG230

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Description
Catalogue Number	AG230
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Glial Fibrillary Acidic Protein, porcine
Overview	Purified porcine Glial Fibrillary Acidic Protein (GFAP) purified from spinal cord.
Alternate Names	GFAP
Background Information	Glial fibrillary acidic protein (UniProt F1RR02) is encoded by the GFAP gene (Gene ID 396562) in porcine. GFAP is a type III intermediate filament (IF) thought to help maintain astrocyte mechanical strength and is closely related to vimentin, desmin, and peripherin, which are involved in the structure and function of cellular cytoskeleton. GFAP is expressed in astrocytes in the central nervous system (CNS), where it is involved in many important CNS processes, including cell communication and the blood brain barrier (BBC) function. GFAP contains the characteristic tripartite domain structure among IF members, a central α-helical rod domain (a.a. 73-377) flanked by a non-α-helical N-terminal head (a.a. 1-72) and a C-terminal tail (a.a. 378-432) domain. The head and tail domains are most structurally variable among IF proteins and their contributions to filament assembly vary depending on the IF proteins. For GFAP, the tail domain is reported to be important for filament assembly. The tail domain R416W mutation is linked to the neurological disorder Alexander disease (AxD).

References

Product Information
Presentation	Liquid. Buffer = PBS.

Applications
Key Applications	Western Blotting ELISA Radioimmunoassay
Application Notes	Western blotting Protein standard ELISA Radioimmunoassay Optimal working dilution must be determined by the end user.

Biological Information
Concentration	1 mg/mL
Purity	Contains >99% pure GFAP, a 50 kDa single band by SDS-PAGE.
Entrez Gene Number	NM_001244397
Gene Symbol	GFAP FLJ45472
UniProt Number	F1RR02
Molecular Weight	49.44 kDa (428 a.a. spliced isoform; NP_001231326) and 53.63 kDa (468 a.a. spliced isoform; XP_005668766) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.