Ms X GluR2

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R, Mk, Ca	ELISA, ICC, IHC, IP, RIA, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R, Mk, Ca

ELISA, ICC, IHC, IP, RIA, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB397
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
Alternate Names	GluR2
Background Information	Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Description

Catalogue Number

MAB397

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4

Alternate Names

GluR2

Background Information

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Product Information
Format	Purified
Control	Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
Presentation	Purified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative.

Product Information

Format

Purified

Control

Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.

Presentation

Purified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative.

Applications
Application	Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.
Key Applications	ELISA Immunocytochemistry Immunohistochemistry Immunoprecipitation Radioimmunoassay Western Blotting
Application Notes	Immunocytochemistry: on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001). ELISA/RIA: A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996). Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002). Immunoprecipitation: 2-4 µg/mL (Osten, 1998). Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals. Optimal working dilutions and protocols must be determined by end user.

Applications

Application

Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.

Key Applications

ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Radioimmunoassay
Western Blotting

Application Notes

Immunocytochemistry:
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).

ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).

Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).

Immunoprecipitation: 2-4 µg/mL (Osten, 1998).

Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.

Optimal working dilutions and protocols must be determined by end user.

Biological Information
Immunogen	Recombinant fusion protein TrpE-GluR2 (putative N-terminal portion, amino acids 175-430).
Epitope	Extracellular
Clone	6C4
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).
Isotype	IgG2a
Species Reactivity	Mouse Rat Monkey Canine
Species Reactivity Note	Expected to react with rat, mouse and canine based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_058957
Entrez Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
Gene Symbol	Gria2 GLUR2 GLURB GluR-2 GluR-B GluR-K2
Purification Method	Purified immunoglobulin from culture supernatant
UniProt Number	P19491
UniProt Summary	FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 883 amino acids; 98,688 Da SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression. TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells. SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. ...
Molecular Weight	102 kDa

Biological Information

Immunogen

Recombinant fusion protein TrpE-GluR2 (putative N-terminal portion, amino acids 175-430).

Epitope

Extracellular

Clone

6C4

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).

Isotype

IgG2a

Species Reactivity

Mouse
Rat
Monkey
Canine

Species Reactivity Note

Expected to react with rat, mouse and canine based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_058957

Entrez Gene Summary

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.

Gene Symbol

Gria2
GLUR2
GLURB
GluR-2
GluR-B
GluR-K2

Purification Method

Purified immunoglobulin from culture supernatant

UniProt Number

P19491

UniProt Summary

FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 883 amino acids; 98,688 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.
TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.
SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. ...

Molecular Weight

102 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on mouse brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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