Anti-PSGL-1 Clone KPL-1 (Mouse Monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IHC, IH(P), IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, IHC, IH(P), IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB4092
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
Alternate Names	PSGL-1 CD162
Background Information	Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

Description

Catalogue Number

MAB4092

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Alternate Names

PSGL-1
CD162

Background Information

Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

Product Information
Format	Purified
Presentation	Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.

Product Information

Format

Purified

Presentation

Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.

Applications
Application	Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
Key Applications	Flow Cytometry Immunohistochemistry Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000 FACS {Snapp, KR et al 1998}. Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein). Functional blocking of P-selectin {Snapp KR et al 1998}. Immunohistochemistry: Fresh frozen, acetone fixed sections Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200 Optimal working dilutions must be determined by the end user.

Applications

Application

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

Key Applications

Flow Cytometry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting

Application Notes

Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

FACS {Snapp, KR et al 1998}.

Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

Functional blocking of P-selectin {Snapp KR et al 1998}.

Immunohistochemistry: Fresh frozen, acetone fixed sections

Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
Clone	KPL-1
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003006.3
Entrez Gene Summary	SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
Gene Symbol	SELPLG CLA PSGL-1 CD162 PSGL1
UniProt Number	Q14242
UniProt Summary	FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells. SIZE: 412 amino acids; 43201 Da SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes. PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.

Biological Information

Immunogen

The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).

Clone

KPL-1

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.

Isotype

IgG1κ

Species Reactivity

Human

Species Reactivity Note

Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_003006.3

Entrez Gene Summary

SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.

Gene Symbol

SELPLG
CLA
PSGL-1
CD162
PSGL1

UniProt Number

Q14242

UniProt Summary

FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells.
SIZE: 412 amino acids; 43201 Da
SUBUNIT: Homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes.
PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C in undiluted aliquots up to 12 months.

Storage and Shipping Information

Storage Conditions

Maintain at 2-8°C in undiluted aliquots up to 12 months.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PSGL-1 Clone KPL-1 (Mouse Monoclonal)

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