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Ms X ACTH

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货号：CBL57

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R ELISA, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number CBL57
Replaces CBL58
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Adrenocorticotropic Hormone Antibody, NT, clone 57
Alternate Names
  • ACTH
References
Product Information
Format Purified
Presentation The monoclonal is presented as 1mg in 1ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Applications
Application This Anti-Adrenocorticotropic Hormone Antibody, N-terminus, clone 57 is validated for use in ELISA, IH(P) for the detection of Adrenocorticotropic Hormone.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
Application Notes This antibody reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen and as such is suitable for serum assay development

The antibody is also reactive on formalin fixed paraffin wax embedded tissue and is therefore suitable for immunocyto-chemistry

Optimal working dilutions must be determined by the end user.
Biological Information
Epitope N-terminus
Clone 57
Host Mouse
Specificity This antibody is specific for Synacthen (1-24 ACTH) and has no cross reaction with CLIP (ACTH 17-39). The antibody has an affinity constant greater than 10E11 L/mol.

KNOWN SPECIES CROSS REACTIVITY: Positive with rat N-terminal ACTH peptide
Isotype IgG1
Species Reactivity
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Gene Symbol
  • POMC
  • pro-ACTH-endorphin
  • adrenocorticotropin
  • beta-LPH
  • POC
  • gamma-LPH
  • LPH
  • ACTH
  • corticotropin
  • beta-endorphin
  • beta-MSH
  • alpha-MSH
  • pro-opiomelanocortin
  • proopiomelanocortin
  • Pro-opiomelanocortin
  • corticotropin-lipotrophin
  • NPP
  • MSH
  • met-enkephalin
  • CLIP
  • gamma-MSH
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.
SIZE: 267 amino acids; 29424 Da
TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.
DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.
SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size 1 mg
Transport Information
Supplemental Information
Specifications

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