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Rb X Glycine Receptor

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货号：AB5052

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5052
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Glycine Receptor Antibody
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Lyophilized from ammonium bicarbonate (5mM) some residual salt may be present. Reconstitute with 100 μL of PBS. Contains no preservatives.
Applications
Application Anti-Glycine Receptor Antibody detects level of Glycine Receptor & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Western blot: 1:1000 using ECL

Immunoprecipitation: 1:10 following the protocol of Raymond et al., Nature (1993) 361:637-641.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Peptide from the amino terminus of the human alpha1 glycine receptor subunit conjugated to KLH.
Host Rabbit
Specificity Recognizes glycine receptor alpha1. Shows cross reactivity to glycine receptor alpha2. subunits The antibody has been used successfully in Western blot and immunoprecipitation assays with cell extracts containing recombinant human glycine receptors and with homogenates of mouse spinal cord. By Western blot the antibody recognizes a protein with a molecular weight of ~ 48 kD.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The inhibitory glycine receptor mediates postsynaptic inhibition in the spinal cord and other regions of the central nervous system. It is a pentameric receptor composed of alpha and beta subunits. The GLRB gene (MIM 138492) encodes the beta subunit of the receptor.[supplied by OMIM]
Gene Symbol
  • GLRA1
  • MGC138878
  • STHE
  • MGC138879
UniProt Number
UniProt Summary FUNCTION: SwissProt: P23415 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
SIZE: 457 amino acids; 52624 Da
SUBUNIT: Pentamer composed of alpha and beta subunits.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P23415 # Defects in GLRA1 are a cause of startle disease (STHE) [MIM:149400]; also known as hereditary hyperekplexia or congenital stiff-person syndrome. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Inheritance can be autosomal dominant or recessive.
SIMILARITY: SwissProt: P23415 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
MISCELLANEOUS: The alpha subunit binds strychnine.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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