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Rb X Rat NET

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货号：AB5066P

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R ELISA, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5066P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Norepinephrine Transporter Antibody
Alternate Names
  • NET
References
Product Information
Format Affinity Purified
Control
  • Brain tissue
Presentation Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA .
Applications
Application This Anti-Norepinephrine Transporter Antibody is validated for use in ELISA, WB for the detection of Norepinephrine Transporter.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL (Chemiluminescence technique): NET can exist is at least two forms, a highly glycosylated ~80kDa form is the most common in brain, and a lessly glycosylated 54kDa form is also seen in some regions and tissues.



ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide/well) Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A 22 amino acid peptide sequence mapping to the 1st extracellular domain of rat NET.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Specific for Norepinephrine Transporter (NET). Norepinephrine Transporter is a 617 amino acid long integral membrane protein belonging to the Sodium:Neurotransmitter Symporter family (Bruss et al. 1995; Melikian et al. 1994; Pacholczyk et al. 1991) The immunogen peptide shows no significant homology with other other known proteins.
Species Reactivity
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The SLC6A2 gene encodes a norepinephrine (noradrenaline) transporter, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis (Kim et al., 2006 [PubMed 17146058]).[supplied by OMIM]
Gene Symbol
  • SLC6A2
  • NAT1
  • NET
  • SLC6A5
  • NET1
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P23975 # Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
SIZE: 617 amino acids; 69332 Da
SUBUNIT: Interacts with PRKCABP.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P23975 # Defects in SLC6A2 are a cause of orthostatic intolerance (OI) [MIM:604715]. OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high.
SIMILARITY: SwissProt: P23975 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Molecular Weight 80 & 54 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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