Ms X Hu CD41 FITC Conjugated

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Bab	FC, IF	M	FITC	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Bab

FC, IF

FITC

Monoclonal Antibody

Description
Catalogue Number	CBL589F
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Integrin αIIb Subunit Antibody, clone PM6/248, FITC conjugated
Alternate Names	CD41

Description

Catalogue Number

CBL589F

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Integrin αIIb Subunit Antibody, clone PM6/248, FITC conjugated

Alternate Names

CD41

Product Information
Format	FITC
Presentation	The monoclonal is presented at a concentration of 100 tests/1 mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Product Information

Format

FITC

Presentation

The monoclonal is presented at a concentration of 100 tests/1 mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Applications
Application	This Anti-Integrin αIIb Subunit Antibody, clone PM6/248, FITC conjugated is validated for use in FC, IF for the detection of Integrin αIIb Subunit.
Key Applications	Flow Cytometry Immunofluorescence
Application Notes	Identification of the alpha-integrin chain of gpIIb/IIa expression on platelets, megakaryocytes and monocytes by indirect immunofluorescence staining Acute leukaemia phenotyping Studies of Glanzmann's thrombasthenia where gpIIb/IIIa is absent or significantly reduced Ligand binding occurs in part via an RGD sequence which is known to be of importance for certain other adhesion interactions with extracellular proteins Optimal working dilutions must be determined by the end user.

Applications

Application

This Anti-Integrin αIIb Subunit Antibody, clone PM6/248, FITC conjugated is validated for use in FC, IF for the detection of Integrin αIIb Subunit.

Key Applications

Flow Cytometry
Immunofluorescence

Application Notes

Identification of the alpha-integrin chain of gpIIb/IIa expression on platelets, megakaryocytes and monocytes by indirect immunofluorescence staining

Acute leukaemia phenotyping

Studies of Glanzmann's thrombasthenia where gpIIb/IIIa is absent or significantly reduced

Ligand binding occurs in part via an RGD sequence which is known to be of importance for certain other adhesion interactions with extracellular proteins

Optimal working dilutions must be determined by the end user.

Biological Information
Clone	PM6/248
Host	Mouse
Specificity	The antibody recognizes the human CD41 cell surface antigen, a 140kDa glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as alpha receptor for fibrinogen, fibronectin and Von Willebrand Factor. It has not been established if clone PM6/248 recognises free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex. FUSION PARTNER: SP2/0 myeloma cell line
Isotype	IgG1
Species Reactivity	Human Baboon
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000419.3
Entrez Gene Summary	ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Gene Symbol	ITGA2B CD41B HPA3 GP2B CD41 GPIIb ITGAB GTA
UniProt Number	P08514
UniProt Summary	FUNCTION: SwissProt: P08514 # Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. SIZE: 1039 amino acids; 113391 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. DISEASE: SwissProt: P08514 # Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. This autosomal recessive disorder is the most common inherited disease of platelets. GT is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. SIMILARITY: SwissProt: P08514 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.

Biological Information

Clone

PM6/248

Host

Mouse

Specificity

The antibody recognizes the human CD41 cell surface antigen, a 140kDa glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as alpha receptor for fibrinogen, fibronectin and Von Willebrand Factor. It has not been established if clone PM6/248 recognises free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex.

FUSION PARTNER: SP2/0 myeloma cell line

Isotype

IgG1

Species Reactivity

Human
Baboon

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000419.3

Entrez Gene Summary

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Gene Symbol

ITGA2B
CD41B
HPA3
GP2B
CD41
GPIIb
ITGAB
GTA

UniProt Number

P08514

UniProt Summary

FUNCTION: SwissProt: P08514 # Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
SIZE: 1039 amino acids; 113391 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
DISEASE: SwissProt: P08514 # Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. This autosomal recessive disorder is the most common inherited disease of platelets. GT is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
SIMILARITY: SwissProt: P08514 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Storage and Shipping Information

Storage Conditions

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Packaging Information
Material Size	100 assays

Packaging Information

Material Size

100 assays

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Ms X Hu CD41 FITC Conjugated

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