Anti-Actin, clone C4 Alexa Fluor® 488 conjugated

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Ch	IF	M	AlexaFluor®488	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Ch

AlexaFluor®488

Monoclonal Antibody

Description
Catalogue Number	MAB1501X
Replaces	MAB1501X
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated
Alternate Names	MAB1501 MAB1501R
Background Information	Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).

Description

Catalogue Number

MAB1501X

Replaces

MAB1501X

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated

Alternate Names

MAB1501
MAB1501R

Background Information

Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).

Product Information
Format	AlexaFluor®488
Control	HeLa cells
Presentation	Purified in PBS with 15 mg/mL BSA and 0.05% NaN3, conjugated to Alexa Fluor 488.

Product Information

Format

AlexaFluor®488

Control

HeLa cells

Presentation

Purified in PBS with 15 mg/mL BSA and 0.05% NaN3, conjugated to Alexa Fluor 488.

Applications
Application	Anti-Actin Antibody, clone C4, Alexa Fluor 488 conjugated detects level of Actin & has been published & validated for use in IF.
Key Applications	Immunofluorescence
Application Notes	Immunofluorescence: 1:200-1:1000 Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Actin Antibody, clone C4, Alexa Fluor 488 conjugated detects level of Actin & has been published & validated for use in IF.

Key Applications

Immunofluorescence

Application Notes

Immunofluorescence: 1:200-1:1000

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified chicken gizzard actin (Lessard, 1988).
Epitope	a.a. 50-70
Clone	C4
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	MAB1501X is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Isotype	IgG2bκ
Species Reactivity	Human Chicken
Species Reactivity Note	Reacts with human. Expected to react with chicken based on 100% homology. Other species have not been tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001100.3
Entrez Gene Summary	Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Gene Symbol	ACTA1 MPFD ASMA NEM1 CFTDM ACTA CFTD NEM2 Alpha-actin-1 CFTD1 NEM3
Purification Method	Protein G Purified
UniProt Number	P68133 P62736 P60709 P63261 P63267 P68032
UniProt Summary	FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SIZE: 377 amino acids; 42051 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. SIMILARITY: SwissProt: P68133 ## Belongs to the actin family. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Molecular Weight	~43 kDa

Biological Information

Immunogen

Purified chicken gizzard actin (Lessard, 1988).

Epitope

a.a. 50-70

Clone

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

MAB1501X is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).

Isotype

IgG2bκ

Species Reactivity

Human
Chicken

Species Reactivity Note

Reacts with human. Expected to react with chicken based on 100% homology. Other species have not been tested.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001100.3

Entrez Gene Summary

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Gene Symbol

ACTA1
MPFD
ASMA
NEM1
CFTDM
ACTA
CFTD
NEM2
Alpha-actin-1
CFTD1
NEM3

Purification Method

Protein G Purified

UniProt Number

UniProt Summary

FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Molecular Weight

~43 kDa

Product Usage Statements
Quality Assurance	Evaluatied on a representative lot by immunofluorescence using HeLa cells.
Usage Statement	Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluatied on a representative lot by immunofluorescence using HeLa cells.

Usage Statement

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Actin, clone C4 Alexa Fluor® 488 conjugated

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