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Ms X Type I Hexokinase

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货号：MAB1532

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, M, R ICC, WB M Ascites Monoclonal Antibody
Description
Catalogue Number MAB1532
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Hexokinase Type I Antibody, a.a. 191-209, clone 21
References
Product Information
Format Ascites
Applications
Application Anti-Hexokinase Type I Antibody, a.a. 191-209, clone 21 is an antibody against Hexokinase Type I for use in IC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application Notes Western blot: 1:500-1:2,000. Immunolocalization of Type I hexokinase: 1:500-1:2,000. Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Rat Type I hexokinase.
Epitope a.a. 191-209
Clone 21
Host Mouse
Specificity Recognizes a segmental epitope located between residues 191-209 of the Type I hexokinase sequence.
Isotype IgG1
Species Reactivity
  • Bovine
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Summary Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
UniProt Number
UniProt Summary SIZE: 917 amino acids; 102486 Da
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding.
TISSUE SPECIFICITY: Isoform 2 is erythrocyte specific; isoform 3 and isoform 4 are testis-specific.
DOMAIN: SwissProt: P19367 The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
DISEASE:SwissProt: P19367 # Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.
SIMILARITY: Belongs to the hexokinase family.
MISCELLANEOUS: In vertebrates there are four major glucose- phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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