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Anti-Neurofilament H&M, phosphorylated, clone NP1

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货号：MAB1592

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Ch, H, R ELISA, FC, RIA, WB, ICC, IH(P) M Ascites Monoclonal Antibody
Description
Catalogue Number MAB1592
Replaces 04-1061
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1
Background Information Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
References
Product Information
Format Ascites
Presentation Liquid. Contains no preservative.
Applications
Application Detect Neurofilament H & M using this Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 validated for use in ELISA, FC, RIA, WB, IC, IH(P).
Key Applications
  • ELISA
  • Flow Cytometry
  • Radioimmunoassay
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application Notes Western blot : recognizes, 200kDa, and 150kDa NF-H and NF-M bands primarily. Staining is removed with phosphatase treatment. Because NF-L also contains lysine-serine PO4 sites, NP1 may cross react with NF-L as well, but this has not be substantiated in traditional westerns.

Immunohistochemistry. Works on paraffin embedded, formalin fixed and frozen acteone fixed tissue sections. 1:50-1:500 for immunofluorescence.

Immunocytochemistry on PC12, TERA-2 and other neuronal lines.

FACS ELISA/RIA

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Bovine neurofilaments
Epitope phosphorylated
Clone NP1
Host Mouse
Specificity Recognizes the KSP repeats of the phosphorylated forms of neurofilament-M (NF-M) and neurofilament-H (NF-H), because NF-L does contain a few of these Lysine-serine-PO4-proline repeats some cross reactivity to NF-L may be seen at very high antibody concentrations.
Isotype IgG1
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEFH
  • NFH
  • NF-H
  • KIAA0845
Modifications
  • Phosphorylation
UniProt Number
UniProt Summary FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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