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Qiagen 凯杰 Biosharp Omega

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Ms Anti-Intergin ß1 Preservative-Free

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号:MAB2259Z

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC, IHC, IP, FUNC, CULT, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB2259Z
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Integrin β1 Antibody, activated, clone B44, azide free
Alternate Names
  • Ligand-inducedBindingSite
  • LIBS
  • CLIBS
  • MAB2259
References
Product Information
Format Purified
Control
  • Human lung, liver, and skeletal muscle tissues
Presentation Purified immunoglobulin. Liquid PBS, containing no preservatives.
Applications
Application This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
  • Cell Culture
  • Western Blotting
Application Notes Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP

Western blot: 10-15 μg/mL

Flow Cytometry: 10-15 μg/mL

Immunohistochemistry

Stimulation of (1-mediated adhesion: 10-20 μg/mL.

Optimal working dilutions must be determined by end user.
Biological Information
Epitope activated, azide free
Clone B44
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAMB2
  • S-laminin
  • LAMS
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SIZE: 1798 amino acids; 195981 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin).
SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction.
DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
Molecular Weight 88 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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