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Ms X Huprolyl 4-hydroxylase

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货号：MAB2701

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number MAB2701
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Prolyl 4-hydroxylase β Antibody, clone 3-2B12
References
Product Information
Format Purified
Presentation Purified immunoglobulin
Applications
Application Detect Prolyl 4-hydroxylase β using this Anti-Prolyl 4-hydroxylase β Antibody, clone 3-2B12 validated for use in ELISA, WB, IH(P).
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunoblotting: 2.5 μg/mL

Immunohistochemistry on frozen and paraffin-embedded tissue sections: 20 μg/mL

ElA

Optimal working dilutions must be determined by the end user.
Biological Information
Clone 3-2B12
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity The antibody specifically reacts with the beta-subunit of hPH. This is a purified mouse monoclonal antibody to human prolyl 4-hydroxylase (hPH).
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene Symbol
  • P4HA1
  • P4HA
  • EC 1.14.11.2
UniProt Number
UniProt Summary FUNCTION: SwissProt: P13674 # Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
COFACTOR: Iron. & Ascorbate.
SIZE: 534 amino acids; 61049 Da
SUBUNIT: Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.
SIMILARITY: SwissProt: P13674 ## Contains 1 PKHD (prolyl/lysyl hydroxylase) domain. & Contains 1 TPR repeat.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • Manufactured by Daiichi Fine Chemical Co., Ltd
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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