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Bovine Angiotensin Converting Enzyme

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货号：AG796

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Uni Prot Number
PC Bovine P12820
Description
Catalogue Number AG796
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Angiotensin Converting Enzyme, bovine
Overview ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase (Kozlowski et al., 1992). ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vasodepressor. Thus, ACE appears to play a key role in regulating vascular tone and remodeling as well as in the development of atherosclerotic lesions. Increasing evidence asuggests that ACE plays an important role in vascular pathology.
Recent studies show two new functions for this important enzyme: 1) ACE enhances presentation of endogenous antigens (such as HIV gp160-derived peptide p18) to MHC class I-restricted T-lymphocytes (Kozlowski et al., 1992); 2) amino-terminal domain of ACE efficiently cleaves hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (Reiger et al., 1993; Rousseau et al., 1995) which is involved in the control of haemopoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (Bogden, 1991).
Alternate Names
  • ACE
  • CD143
  • DCP1
  • Peptidyl-dipeptidase A
  • Peptidase P
References
Product Information
Applications
Key Applications
  • Positive Control
Application Notes 1) As a reference in SDS-PAGE and Western blotting in combination with anti-ACE MABs (MAB3500 and MAB3502). For Western with anti-ACE monoclonal antibodies load 20 ng/lane.
2) As a reference antigen for immunoprecipitation of ACE from various tissue samples.
3) For enzymatic studies (substrate specificity, enzyme kinetics, etc.).

Optimal working concentrations must be determined by the end user.
Biological Information
Concentration See lot specific Datasheet for specific concentration and activity.
Purity >95% as determined by SDS-PAGE.
Source Isolated from bovine lung membrane by lisinopril-affinity chromatography (ref 5).
Specific Activity One unit produces 1 mmole of hippuric acid or His-Leu from Hippuril-His-Leu per min in 0.1 M phosphate buffer-300 mM NaCl, pH 8.3 at 37&deg:C. <br /><br />pH-OPTIMUM: pH 7.5-8.5
Gene Symbol
  • ACE
  • DCP1
UniProt Number
Molecular Weight 170 kDa under reducing and non-reducing conditions.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months. Maintain at 4°C for up to one month. A decrease in activity may occur within prolonged storage at 4°C.
Packaging Information
Material Size 1 units
Transport Information
Supplemental Information
Specifications

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