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Rabbit Anti-HCN-1 Affinity Purified Polyclonal Antibody

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货号：AB5884-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5884-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-HCN1 Antibody
Alternate Names
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1
  • HAC2
  • BCNG1
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application Anti-HCN1 Antibody is an antibody against HCN1 for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:200-1:400 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide corresponding amino acids 6-24 of rat HCN-1 (Accession number AAF62173).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes HCN-1 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1). The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse (18/19) and possibly rabbit (16/19) due to sequence homology. Other species have not been tested.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]
Gene Symbol
  • HCN1
  • HAC-2
  • BCNG1
  • BCNG-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: O60741 # Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.
SIZE: 890 amino acids; 98929 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2 (By similarity). Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
DOMAIN: SwissProt: O60741 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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