GP X ASIC3

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	ICC, IHC	Gp	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ICC, IHC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5927
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3
Alternate Names	ASIC3 Amiloride-sensitive Cation Channel 3 TNaC1 Testis Sodium Channel 1 Dorsal Root Ganglion ASIC (DRASIC)

Description

Catalogue Number

AB5927

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3

Alternate Names

ASIC3
Amiloride-sensitive Cation Channel 3
TNaC1
Testis Sodium Channel 1
Dorsal Root Ganglion ASIC (DRASIC)

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin. Liquid in PBS, pH 7.4 with 1% BSA and 0.05% sodium azide.

Product Information

Format

Affinity Purified

Presentation

Affinity purified immunoglobulin. Liquid in PBS, pH 7.4 with 1% BSA and 0.05% sodium azide.

Applications
Application	Detect Acid Sensitive Ion Channel 3 using this Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3 validated for use in IC, IH.
Key Applications	Immunocytochemistry Immunohistochemistry
Application Notes	Immunohistochemistry: 1:100 Immunocytochemistry: 1:100 Optimal working dilutions must be determined by the end user. APPLICATION NOTES FOR AB5927 IMMUNOHISTOCHEMISTRY Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Applications

Application

Detect Acid Sensitive Ion Channel 3 using this Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3 validated for use in IC, IH.

Key Applications

Immunocytochemistry
Immunohistochemistry

Application Notes

Immunohistochemistry: 1:100

Immunocytochemistry: 1:100

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5927

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Biological Information
Immunogen	Synthetic peptide from the extracellular domain of rat ASIC3 (Catalog Number AG366). (C)TASLDPDDFDPEPSDPLGSP
Epitope	285-304 of extracellular domain of rat ASIC3
Host	Guinea Pig
Specificity	ASIC3 (DRASIC)
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004769.1 NM_020321.1 NM_020322.1
Entrez Gene Summary	This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Alternative splicing of this gene generates three transcript variants encoding distinct isoforms.
Gene Symbol	ACCN3 ASIC3 hTNaC1 TNaC1 TNAC1 hASIC3 SLNAC1
UniProt Number	Q9UHC3
UniProt Summary	FUNCTION: SwissProt: Q9UHC3 # Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. SIZE: 531 amino acids; 58905 Da SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4 (By similarity). Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Cytoplasm (By similarity). Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity).TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues.DOMAIN:SwissProt: Q9UHC3 The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1. PTM: Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel (By similarity). SIMILARITY: Belongs to the amiloride-sensitive sodium channel family. MISCELLANEOUS: Potentiated by FMRFamide-related neuropeptides. Sensitized and potentiated by NPSF. Regulated by lactate and Ca(2+). Inhibited by anti-inflammatory drugs, like salicylic acid (By similarity). Sensitized and potentiated by NPFF.

Biological Information

Immunogen

Synthetic peptide from the extracellular domain of rat ASIC3 (Catalog Number AG366). (C)TASLDPDDFDPEPSDPLGSP

Epitope

285-304 of extracellular domain of rat ASIC3

Host

Guinea Pig

Specificity

ASIC3 (DRASIC)

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Alternative splicing of this gene generates three transcript variants encoding distinct isoforms.

Gene Symbol

ACCN3
ASIC3
hTNaC1
TNaC1
TNAC1
hASIC3
SLNAC1

UniProt Number

Q9UHC3

UniProt Summary

FUNCTION: SwissProt: Q9UHC3 # Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
SIZE: 531 amino acids; 58905 Da
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4 (By similarity). Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Cytoplasm (By similarity). Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity).TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues.DOMAIN:SwissProt: Q9UHC3 The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1.
PTM: Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel (By similarity).
SIMILARITY: Belongs to the amiloride-sensitive sodium channel family.
MISCELLANEOUS: Potentiated by FMRFamide-related neuropeptides. Sensitized and potentiated by NPSF. Regulated by lactate and Ca(2+). Inhibited by anti-inflammatory drugs, like salicylic acid (By similarity). Sensitized and potentiated by NPFF.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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