Anti-Potassium Channel KvLQT1, C-terminus (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IHC, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5932
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Potassium Channel KvLQT1 Antibody, CT
Alternate Names	IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 KQT-like 1 Voltage-gated potassium channel subunit Kv7.1 kidney and cardiac voltage dependent K+ channel potassium voltage-gated channel, KQT-like subfamily, member 1 slow delayed rectifier channel subunit
Background Information	KvLQT1(Kv7.1) is a voltage-gated potassium channel protein coded for by the gene KCNQ1. This protein is present in the cell membranes of cardiac muscle tissue and in inner ear neurons among other tissues. KvLQT1 can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3 and in heart cells is required for the repolarization phase of the cardiac action potential. Mutations in the gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation, lead to a defective KvLQT1 protein and several forms of inherited arrhythmias known as Long QT syndrome, Short QT syndrome, and Familial Atrial Fibrillation.

Description

Catalogue Number

AB5932

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Potassium Channel KvLQT1 Antibody, CT

Alternate Names

IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
KQT-like 1
Voltage-gated potassium channel subunit Kv7.1
kidney and cardiac voltage dependent K+ channel
potassium voltage-gated channel, KQT-like subfamily, member 1
slow delayed rectifier channel subunit

Background Information

KvLQT1(Kv7.1) is a voltage-gated potassium channel protein coded for by the gene KCNQ1. This protein is present in the cell membranes of cardiac muscle tissue and in inner ear neurons among other tissues. KvLQT1 can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3 and in heart cells is required for the repolarization phase of the cardiac action potential. Mutations in the gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation, lead to a defective KvLQT1 protein and several forms of inherited arrhythmias known as Long QT syndrome, Short QT syndrome, and Familial Atrial Fibrillation.

Product Information
Format	Affinity Purified
Control	Human heart tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Human heart tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Anti-Potassium Channel KvLQT1 Antibody, C-terminus detects level of Potassium Channel KvLQT1 & has been published & validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Potassium Channel KvLQT1 in human myocardium tissue. /Western Blotting Analysis: 2 μg/mL of this antibody detected KvLQT1 in 10 μg of human heart tissue lysate. Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Potassium Channel KvLQT1 Antibody, C-terminus detects level of Potassium Channel KvLQT1 & has been published & validated for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Potassium Channel KvLQT1 in human myocardium tissue.

/Western Blotting Analysis: 2 μg/mL of this
antibody detected KvLQT1 in 10 μg of human
heart tissue lysate.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	KLH-conjugated linear peptide from the C-terminus of human KvLQT1.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Potassium Channel KvLQT1 protein.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Demonstrated to react with human. Predicted to react with rat and mouse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000209.2
Entrez Gene Summary	This gene encodes a protein for a voltage-gated potassium channel required for the repolarization phase of the cardiac action potential. The gene product can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation. The gene is located in a region of chromosome 11 that contains a large number of contiguous genes that are abnormally imprinted in cancer and the Beckwith-Wiedemann syndrome. Two alternative transcripts encoding distinct isoforms have been described.
Gene Symbol	KCNQ1 JLNS1 ATFB1 SQT2 CNA8 LQT WRS Kv1.9 KVLQT1 RWS Kv7.1 KCNA9 QTS LQT1
Purification Method	Affinity Purfied
UniProt Number	P51787
UniProt Summary	FUNCTION: Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current in CHO cells in which cloned KCNQ1/KCNE1 channels were coexpressed with M1 muscarinic receptors. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea. SIZE: 676 amino acids; 74699 Da SUBUNIT: Heteromultimer with KCNE1 (MinK) or KCNE3 (MiRP2). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. DISEASE: Defects in KCNQ1 are the cause of long QT syndrome type 1 (LQT1) [MIM:192500]; also known as Romano-Ward syndrome (RWS). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. LQT1 inheritance is an autosomal dominant. & Defects in KCNQ1 are a cause of Jervell and Lange-Nielsen syndrome (JLNS) [MIM:220400]. JLNS is an autosomal recessive cardio-auditory syndrome characterized by a prolonged QT interval in the electrocardiogram and congenital deafness. & Defects in KCNQ1 are the cause of atrial fibrillation type 1 (ATFB1) [MIM:607554]. ATFB is characterized by rapid and irregular activation of the atrium. ATFB causes thromboembolism, tachycardia-mediated cardiomyopathy, heart failure and ventricular arrhythmia. & Defects in KCNQ1 are the cause of short QT syndrome type 2 (SQT2) [MIM:609621]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SIMILARITY: Belongs to the potassium channel family. KQT subfamily. MISCELLANEOUS: Mutagenesis experiments were carried out by expressing in Xenopus oocytes or COS-7 cells KCNQ1 mutants either individually (homomultimers) or in combination with both wild-type KCNQ1 (mut/wt homomultimers) and minK (heteromultimers).
Molecular Weight	~ 72 kDa Observed

Biological Information

Immunogen

KLH-conjugated linear peptide from the C-terminus of human KvLQT1.

Epitope

C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes Potassium Channel KvLQT1 protein.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Demonstrated to react with human. Predicted to react with rat and mouse based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_000209.2

Entrez Gene Summary

This gene encodes a protein for a voltage-gated potassium channel required for the repolarization phase of the cardiac action potential. The gene product can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation. The gene is located in a region of chromosome 11 that contains a large number of contiguous genes that are abnormally imprinted in cancer and the Beckwith-Wiedemann syndrome. Two alternative transcripts encoding distinct isoforms have been described.

Gene Symbol

KCNQ1
JLNS1
ATFB1
SQT2
CNA8
LQT
WRS
Kv1.9
KVLQT1
RWS
Kv7.1
KCNA9
QTS
LQT1

Purification Method

Affinity Purfied

UniProt Number

P51787

UniProt Summary

FUNCTION: Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current in CHO cells in which cloned KCNQ1/KCNE1 channels were coexpressed with M1 muscarinic receptors. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea.

SIZE: 676 amino acids; 74699 Da

SUBUNIT: Heteromultimer with KCNE1 (MinK) or KCNE3 (MiRP2).

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

TISSUE SPECIFICITY: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.

DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

DISEASE: Defects in KCNQ1 are the cause of long QT syndrome type 1 (LQT1) [MIM:192500]; also known as Romano-Ward syndrome (RWS). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. LQT1 inheritance is an autosomal dominant. & Defects in KCNQ1 are a cause of Jervell and Lange-Nielsen syndrome (JLNS) [MIM:220400]. JLNS is an autosomal recessive cardio-auditory syndrome characterized by a prolonged QT interval in the electrocardiogram and congenital deafness. & Defects in KCNQ1 are the cause of atrial fibrillation type 1 (ATFB1) [MIM:607554]. ATFB is characterized by rapid and irregular activation of the atrium. ATFB causes thromboembolism, tachycardia-mediated cardiomyopathy, heart failure and ventricular arrhythmia. & Defects in KCNQ1 are the cause of short QT syndrome type 2 (SQT2) [MIM:609621]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.

SIMILARITY: Belongs to the potassium channel family. KQT subfamily.

MISCELLANEOUS: Mutagenesis experiments were carried out by expressing in Xenopus oocytes or COS-7 cells KCNQ1 mutants either individually (homomultimers) or in combination with both wild-type KCNQ1 (mut/wt homomultimers) and minK (heteromultimers).

Molecular Weight

~ 72 kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human heart tissue lysate. Western Blot Analysis: 2 µg/ml of this antibody detected KvLQT1 in 10 µg of human heart tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human heart tissue lysate.

Western Blot Analysis: 2 µg/ml of this antibody detected KvLQT1 in 10 µg of human heart tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Potassium Channel KvLQT1, C-terminus (rabbit polyclonal)

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询