Anti-ROK?/ROCK-II, clone A9W4 (rabbit monoclonal) - 炼石商城

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Anti-ROK?/ROCK-II, clone A9W4 (rabbit monoclonal)

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货号：04-841

品牌：Millipore 密理博

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北京朝阳

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, B	IP, WB	Rb	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	04-841
Replaces	05-841
Description	Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
Alternate Names	RhoA-binding kinase 2 Rho-associated, coiled-coil containing protein kinase 2 Rho-associated, coiled-coil-containing protein kinase II p150 ROK-alpha p164 ROCK-2 ROCK-II ROKalpha Rock2
Background Information	Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

References

Product Information
Format	Culture Supernatant
Control	RIPA lysates from PC-12 cells
Presentation	Cultured supernatant containing 0.05% sodium azide.

Applications
Application	Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Key Applications	Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

Biological Information
Immunogen	6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
Clone	A9W4
Host	Rabbit
Specificity	Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
Species Reactivity	Human Mouse Rat Bovine
Species Reactivity Note	Human and rat. Predicted to cross-react with mouse and bovine based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_013022
Entrez Gene Summary	A Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD].
Gene Symbol	EC 2.7.11.1 MGC131603 MGC43611 OTTHUMP00000162514 P160ROCK PRO0435
UniProt Number	Q62868
UniProt Summary	FUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis By similarity. Catalytic activity ATP + a protein = ADP + a phosphoprotein. Enzyme regulation Activated by RHOA binding. SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM By similarity. Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2. SUBCELLULAR LOCATION: Cytoplasm. Centrosome › centrioleBy similarity. Golgi apparatus membrane; Peripheral membrane protein. Note= Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes. TISSUE SPECIFICITY: Detected in blood platelets. Ref.1 DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.1 Ref.10 Ref.11 Ref.12 Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. Miscellaneous Inhibited by Y-27632. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat.
Molecular Weight	150 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells. Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	200 µL

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