Anti-TBK1, clone AOW9 (rabbit monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Ca, Ch	WB, IP	Rb	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Ca, Ch

WB, IP

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	04-856
Replaces	05-856
Description	Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal
Alternate Names	NF-kB-activating kinase NF-kappa-B-activating kinase TANK-binding kinase 1
Background Information	TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

Description

Catalogue Number

04-856

Replaces

05-856

Description

Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal

Alternate Names

NF-kB-activating kinase
NF-kappa-B-activating kinase
TANK-binding kinase 1

Background Information

TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

Product Information
Format	Culture Supernatant
Control	RIPA lysates from HEK293 cells.
Presentation	Cultured supernatant containing 0.05% sodium azide.

Product Information

Format

Culture Supernatant

Control

RIPA lysates from HEK293 cells.

Presentation

Cultured supernatant containing 0.05% sodium azide.

Applications
Application	Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation: 1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.

Applications

Application

Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.

Biological Information
Immunogen	Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
Epitope	a.a. 642-645
Clone	AOW9
Host	Rabbit
Specificity	TBK1; Does not cross-react with IKKε, IKKα or IKKβ.
Isotype	IgG
Species Reactivity	Human Mouse Canine Chicken
Species Reactivity Note	Human. Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_037386
Entrez Gene Summary	The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq]
Gene Symbol	EC 2.7.11.1 FLJ11330 NAK T2K
UniProt Number	Q9UHD2
UniProt Summary	FUNCTION: Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. Ref.1 Ref.2 Ref.5 Ref.7 Ref.8 Ref.11 Catalytic activity ATP + a protein = ADP + a phosphoprotein. SUBUNIT: Interacts with TIRAP, TANK and TRAF2. Part of a ternary complex consisting of TANK, TRAF2 and TBK1. Interacts with AZI2. Interacts with SIKE. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between TBK1 and SIKE. Interacts with HCV NS3, a hepatitis C virus protein and with BDV P protein, a Borna disease virus protein. Ref.1 Ref.6 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 SUBCELLULAR LOCATION:Cytoplasm. Ref.11 SPECIFICITY:Ubiquitous with higher expression in testis. Ref.2 SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain.
Molecular Weight	85 kDa

Biological Information

Immunogen

Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.

Epitope

a.a. 642-645

Clone

AOW9

Host

Rabbit

Specificity

TBK1; Does not cross-react with IKKε, IKKα or IKKβ.

Isotype

IgG

Species Reactivity

Human
Mouse
Canine
Chicken

Species Reactivity Note

Human. Predicted to cross-react with mouse, dog, and chicken based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_037386

Entrez Gene Summary

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq]

Gene Symbol

EC 2.7.11.1
FLJ11330
NAK
T2K

UniProt Number

Q9UHD2

UniProt Summary

FUNCTION: Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. Ref.1 Ref.2 Ref.5 Ref.7 Ref.8 Ref.11

Catalytic activity ATP + a protein = ADP + a phosphoprotein.

SUBUNIT: Interacts with TIRAP, TANK and TRAF2. Part of a ternary complex consisting of TANK, TRAF2 and TBK1. Interacts with AZI2. Interacts with SIKE. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between TBK1 and SIKE. Interacts with HCV NS3, a hepatitis C virus protein and with BDV P protein, a Borna disease virus protein. Ref.1 Ref.6 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14

SUBCELLULAR LOCATION:Cytoplasm. Ref.11

SPECIFICITY:Ubiquitous with higher expression in testis. Ref.2

SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Contains 1 protein kinase domain.

Molecular Weight

85 kDa

Product Usage Statements
Quality Assurance	Evaluated by western blot on RIPA lysates from HEK293 cells. Western Blot Analysis: A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on RIPA lysates from HEK293 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-TBK1, clone AOW9 (rabbit monoclonal)

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