Anti-Smad2/3, clone C4T (rabbit monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	04-914
Replaces	05-914
Description	Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal
Alternate Names	Deletion target in pancreatic carcinoma 4 MAD, mothers against decapentaplegic homolog 4 MAD, mothers against decapentaplegic homolog 4 (Drosophila) Mothers against DPP homolog 4 SMAD 4 SMAD family member 4 SMAD, mothers against DPP homolog 4 SMAD, mothers against DPP homolog 4 (Drosophila) deleted in pancreatic carcinoma locus 4 mothers against decapentaplegic homolog 4 mothers against decapentaplegic, Drosophila, homolog of, 4
Background Information	Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

Description

Catalogue Number

04-914

Replaces

05-914

Description

Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal

Alternate Names

Deletion target in pancreatic carcinoma 4
MAD, mothers against decapentaplegic homolog 4
MAD, mothers against decapentaplegic homolog 4 (Drosophila)
Mothers against DPP homolog 4
SMAD 4
SMAD family member 4
SMAD, mothers against DPP homolog 4
SMAD, mothers against DPP homolog 4 (Drosophila)
deleted in pancreatic carcinoma locus 4
mothers against decapentaplegic homolog 4
mothers against decapentaplegic, Drosophila, homolog of, 4

Background Information

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

Product Information
Format	Culture Supernatant
Control	RIPA lysates from L6 cells
Presentation	Cultured supernantant containing 0.05% sodium azide.

Product Information

Format

Culture Supernatant

Control

RIPA lysates from L6 cells

Presentation

Cultured supernantant containing 0.05% sodium azide.

Applications
Application	Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4.
Key Applications	Western Blotting

Applications

Application

Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4.

Key Applications

Western Blotting

Biological Information
Immunogen	Fusion protein corresponding to amino acids 186-273 of human Smad2.
Epitope	a.a. 186-273
Clone	C4T
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human. Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001003652.1 NP_005893.1
Entrez Gene Summary	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Gene Symbol	DPC4 JIP MADH4 OTTHUMP00000163548 hSMAD4
UniProt Number	Q13485
UniProt Summary	FUNCTION:Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor. SUBUNIT STRUCTURE: May form trimers with receptor-regulated SMAD (R-SMAD). Found in a ternary complex composed of SMAD4, STK11 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11, STK11IP and TRIM33. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with USP9X. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD. PTM: Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. INVOLVEMENT IN DISEASE: Defects in SMAD4 are a cause of pancreatic carcinoma [MIM:260350]. Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers. Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis (JIP) and hereditary hemorrhagic telangiectasia (HHT) [MIM:187300] in a single individual. JIP and HHT are autosomal dominant disorders with distinct and non-overlapping clinical features. The former, an inherited gastrointestinal malignancy predisposition, is caused by mutations in SMAD4 or BMPR1A, and the latter is a vascular malformation disorder caused by mutations in ENG or ACVRL1. All four genes encode proteins involved in the transforming-growth-factor-signaling pathway. Although there are reports of patients and families with phenotypes of both disorders combined, the genetic etiology of this association is unknown. Defects in SMAD4 may be a cause of colorectal cancer (CRC) [MIM:114500]. SEQUENCE SIMILARITIES:Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.
Molecular Weight	~50-60 kDa

Biological Information

Immunogen

Fusion protein corresponding to amino acids 186-273 of human Smad2.

Epitope

a.a. 186-273

Clone

C4T

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human. Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Entrez Gene Summary

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional
modulators that mediate multiple signaling pathways. This protein
mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is
recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively
spliced transcript variants encoding the same protein have been observed.

Gene Symbol

DPC4
JIP
MADH4
OTTHUMP00000163548
hSMAD4

UniProt Number

Q13485

UniProt Summary

FUNCTION:Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor.
SUBUNIT STRUCTURE: May form trimers with receptor-regulated SMAD (R-SMAD). Found in a ternary complex composed of SMAD4, STK11 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11, STK11IP and TRIM33. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with USP9X.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD.
PTM: Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade.
INVOLVEMENT IN DISEASE: Defects in SMAD4 are a cause of pancreatic carcinoma [MIM:260350].
Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.
Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis (JIP) and hereditary hemorrhagic telangiectasia (HHT) [MIM:187300] in a single individual. JIP and HHT are autosomal dominant disorders with distinct and non-overlapping clinical features. The former, an inherited gastrointestinal malignancy predisposition, is caused by mutations in SMAD4 or BMPR1A, and the latter is a vascular malformation disorder caused by mutations in ENG or ACVRL1. All four genes encode proteins involved in the transforming-growth-factor-signaling pathway. Although there are reports of patients and families with phenotypes of both disorders combined, the genetic etiology of this association is unknown.
Defects in SMAD4 may be a cause of colorectal cancer (CRC) [MIM:114500].
SEQUENCE SIMILARITIES:Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.

Molecular Weight

~50-60 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on L6 lysates. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on L6 lysates.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µl

Packaging Information

Material Size

100 µl

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Smad2/3, clone C4T (rabbit monoclonal)

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