Neural Cell Adhesion Molecule [NCAM]

Key Applications	Entrez Gene Number	Uni Prot Number
WB	XM_001234121.1	P13590

Key Applications

Entrez Gene Number

Uni Prot Number

XM_001234121.1

P13590

Description
Catalogue Number	AG265
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Neural Cell Adhesion Molecule, chicken
Background Information	The neural cell adhesion molecule (NCAM) was the first cell-cell adhesion molecule to be identified, isolated, and sequenced. Structurally, the extracellular portion of NCAM is comprised of five IgG-like domains followed by two fibronectin type III repeats. Because of its history, NCAM is considered to be the classic example of the family of cell-cell adhesion molecules that are structurally related to immunoglobulins. There are three forms of NCAM molecules that differ in their mode of attachment to the plasma membrane: ld, transmembrane form with large cytoplasmic domain; sd, transmembrane form with small cytoplasmic domain; ssd, GPI linked to the external surface of the plasma membrane. NCAM is unique in that the molecule can bear large oligosaccharides containing long chains of polysialic acid. Functionally, NCAM mediates cell-cell adhesion both in neural cells and a variety of other cell types. NCAM-mediated adhesion occurs by both a homophilic mechanism (NCAM to NCAM) and a heterophilic mechanism in which NCAM binds to heparin sulfate proteoglycans. The polysialic acid in NCAM interferes with NCAM-mediated cell-cell adhesion. Moreover, the polysialic acid on NCAM fills up so much of the space between cells that it also interferes with cell-cell adhesion mediated by other adhesion molecules. It has been suggested that the polysialic acid on NCAM is involved in maintaining plasticity in interactions among neural cells. NCAM is expressed at a wide variety of sites during embryonic development both in the nervous system and other tissues. Its expression is more restricted in the adult brain; however, it is still expressed in certain regions. NCAM expression is also associated with certain diseases. In particular, it is considered to be a marker for Wilms tumor and for small cell lung carcinoma. Chemicon's preparation of NCAM is derived from embryonic chicken brains and is composed of polysialic acid-rich molecules. Neuraminidase treatment of the preparation reveals that it contains mainly the ld form of NCAM with a smaller amount of the sd form.

Description

Catalogue Number

AG265

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Neural Cell Adhesion Molecule, chicken

Background Information

The neural cell adhesion molecule (NCAM) was the first cell-cell adhesion molecule to be identified, isolated, and sequenced. Structurally, the extracellular portion of NCAM is comprised of five IgG-like domains followed by two fibronectin type III repeats. Because of its history, NCAM is considered to be the classic example of the family of cell-cell adhesion molecules that are structurally related to immunoglobulins. There are three forms of NCAM molecules that differ in their mode of attachment to the plasma membrane: ld, transmembrane form with large cytoplasmic domain; sd, transmembrane form with small cytoplasmic domain; ssd, GPI linked to the external surface of the plasma membrane. NCAM is unique in that the molecule can bear large oligosaccharides containing long chains of polysialic acid. Functionally, NCAM mediates cell-cell adhesion both in neural cells and a variety of other cell types. NCAM-mediated adhesion occurs by both a homophilic mechanism (NCAM to NCAM) and a heterophilic mechanism in which NCAM binds to heparin sulfate proteoglycans. The polysialic acid in NCAM interferes with NCAM-mediated cell-cell adhesion. Moreover, the polysialic acid on NCAM fills up so much of the space between cells that it also interferes with cell-cell adhesion mediated by other adhesion molecules. It has been suggested that the polysialic acid on NCAM is involved in maintaining plasticity in interactions among neural cells. NCAM is expressed at a wide variety of sites during embryonic development both in the nervous system and other tissues. Its expression is more restricted in the adult brain; however, it is still expressed in certain regions. NCAM expression is also associated with certain diseases. In particular, it is considered to be a marker for Wilms tumor and for small cell lung carcinoma. Chemicon's preparation of NCAM is derived from embryonic chicken brains and is composed of polysialic acid-rich molecules. Neuraminidase treatment of the preparation reveals that it contains mainly the ld form of NCAM with a smaller amount of the sd form.

Product Information
Presentation	Purified NCAM in 15 mM octylthioglucoside/ 0.5 M NaHCO3. Contains no preservative.

Product Information

Presentation

Purified NCAM in 15 mM octylthioglucoside/ 0.5 M NaHCO3. Contains no preservative.

Applications
Key Applications	Western Blotting
Application Notes	Source: Embryonic Chicken Brain. Molecular Weight: 200-250 kDa, when treated with Neurminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

Applications

Key Applications

Western Blotting

Application Notes

Source: Embryonic Chicken Brain. Molecular Weight: 200-250 kDa, when treated with Neurminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

Biological Information
Concentration	0.5 mg/mL
Source	Embryonic Chicken Brain. Molecular Weight: 200-250 kDa, when treated with Neurminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Entrez Gene Number	XM_001234121.1
Gene Symbol	NCAM1 NCAM-120 CD56 NCAM-140 MSK39 NCAM
UniProt Number	P13590
UniProt Summary	FUNCTION: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. SIZE: 1,091 amino acids; 117,415 Da SUBCELLULAR LOCATION: Cell membrane; Single-Pass membrane protein(Potential) SIMILARITY: Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Biological Information

Concentration

0.5 mg/mL

Source

Embryonic Chicken Brain. Molecular Weight: 200-250 kDa, when treated with Neurminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

Entrez Gene Number

XM_001234121.1

Gene Symbol

NCAM1
NCAM-120
CD56
NCAM-140
MSK39
NCAM

UniProt Number

P13590

UniProt Summary

FUNCTION: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
SIZE: 1,091 amino acids; 117,415 Da
SUBCELLULAR LOCATION: Cell membrane; Single-Pass membrane protein(Potential)
SIMILARITY: Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. If required for in vivo applications, detergent can be dialyzed away.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. If required for in vivo applications, detergent can be dialyzed away.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Neural Cell Adhesion Molecule [NCAM]

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