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Ms X Microphthalmia

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货号：MAB3747

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MABE78
Replaces MAB3747
Description Anti-MiTF Antibody, clone 3D1.2
Alternate Names
  • Microphthalmia-associated transcription factor
  • Class E basic helix-loop-helix protein 32
  • bHLHe32
Background Information Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
References
Product Information
Format Purified
Control
  • Mouse brain tissue lysate
Presentation Purified mouse monoclonal IgMκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human MiTF.
Clone 3D1.2
Host Mouse
Isotype IgMκ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MITF
  • BHLHE32
Purification Method Purified by Ion-Exchange Chromatography
UniProt Number
Molecular Weight ~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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