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Anti-Annexin II (mouse monoclonal)

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货号：MAB3774

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, IP, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB3774
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Annexin II Antibody, clone 3D5/4
Alternate Names
  • Lipocortin II
  • Calpactin I Heavy Chain
  • Chromobindin 8
Background Information Annexin II belongs to a family of Ca2+ dependent phospholipid binding proteins. It is thought to serve as a profibrinolytic coreceptor for both plasminogen and tissue plasminogen activator on the surface of endothelial cells. It is also known to interact with viral pathogens.
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
Applications
Application Detect Annexin II using this Anti-Annexin II Antibody, clone 3D5/4 validated for use in ELISA, IP, IH.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Immunohistochemistry
Application Notes Immunoprecipitation

Immunohistochemistry: acetone fixed frozen sections

ELISA

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Whole native annexin proteins contained in a mixture of membrane proteins from human bone tissue
Clone 3D5/4
Host Mouse
Specificity Recognizes human Annexin II
Isotype IgG2a
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • ANXA2
  • LPC2D
  • P36
  • p36
  • Chromobindin-8
  • LIP2
  • Annexin-2
  • ANX2L4
  • LPC2
  • ANX2
  • PAP-IV
  • CAL1H
UniProt Number
UniProt Summary FUNCTION: SwissProt: P07355 # Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
SIZE: 339 amino acids; 38604 Da
SUBUNIT: Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
DOMAIN: SwissProt: P07355 A pair of annexin repeats may form one binding site for calcium and phospholipid.
PTM: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
SIMILARITY: Belongs to the annexin family. & Contains 4 annexin repeats.
MISCELLANEOUS: It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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