Anti-MAP2A, 2B

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货号：MAB378

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
Am, Av, B, H, M, R, Xn, Qu	IHC, WB	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MAB378
Replaces	MAB3418
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MAP2A Antibody, 2B
Alternate Names	Microtubule Associated Protein 2
Background Information	MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984). MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

References

Product Information
Format	Ascites
Control	Brain tissue Cultured neurons
Presentation	Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

Applications
Application	Detect MAP2A using this Anti-MAP2A Antibody, 2B validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry: 1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry. Western blot: SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Western blot: A 1:500 dilution was used in a previous lot. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Bovine MAP2
Clone	AP20
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	MAP2a & b. Does not recognize other variants.
Isotype	IgG1
Species Reactivity	Amphibian Avian Bovine Human Mouse Rat Xenopus Quail
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001039538.1
Entrez Gene Summary	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol	MAP2 MAP2B MAP-2 MAP2C DKFZp686I2148 MAP2A
Purification Method	Unpurified
UniProt Number	P11137
UniProt Summary	FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Molecular Weight	~ 300 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.