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Anti-Human Tissue Transglutaminase (mouse monoclonal)

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货号：MAB3839

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ICC M Purified Monoclonal Antibody
Description
Catalogue Number MAB3839
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3
Alternate Names
  • tTG
  • Protein-glutamine gamma-Glutamyltransferase
  • Transglutaminase 2
  • TGase C
Background Information Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues (Monsonego, 1997). Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation.

High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.
References
Product Information
Format Purified
Presentation Protein A purified immunoglobulin in 0.02M Phosphate Buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Applications
Application Detect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC.
Key Applications
  • Immunocytochemistry
Application Notes Immunohistochemistry: 20 μg/mL

Adhesion Blocking: 10-20 μg/mL (per 2x10e5 cells). 4G3 interferes with adhesion of TPA stimulated THP-1 cells and M-CSF stimulated peripheral blood monocytes to fibronectin.

Optimal working dilutions must be determined by the end user.



PROTOCOL:

IMMUNOCYTOCHEMISTRY: THP-1 cells were induced to differentiate into macrophages by treatment with 150 ng/ml of TPA and plated on fibronectin coated glass coverslips for 72 hours.Live non-permeablized cells were incubated with 20 μg/mL of MAB3839 for 45 minutes, washed three times with PBS and fixed for 10 minutes with 3.7% paraformaldehyde. The cells were then washed with PBS and incubated 45 minutes with a fluoresceine labeled affinity-purified goat-anti-mouse secondary antibody. After extensive washing with PBS, cells were mounted in Fluorsave medium.

Observed Staining:Cell surface tTG was colocalized with b1- and b3-Integrins in podosome-like adhesive structures of macrophages adherent on fibronectin.
Biological Information
Immunogen Fibronectin binding domain of tissue transglutaminase (amino acids 1-165 of tTG).
Epitope FN binding domain
Clone 4G3
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Tissue Transglutaminase. Will recognize all three known splice variants of tTG.
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • TGM2
  • TG
  • TGC
  • TG2
  • C
  • protein-glutamine-gamma-glutamyltransferase
  • Transglutaminase-2
  • TGase-H
  • EC 2.3.2.13
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21980 # Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
SIZE: 687 amino acids; 77329 Da
SUBUNIT: Monomer.
SIMILARITY: SwissProt: P21980 ## Belongs to the transglutaminase superfamily. Transglutaminase family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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