a-JNK3/SAPK1b, clone C05T 100ul

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB	Rb	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	04-893
Replaces	05-893
Description	Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
Alternate Names	JNK3 alpha protein kinase MAP kinase MAP kinase p49 3F12 Stress-activated protein kinase JNK3 c-Jun N-terminal kinase 3 c-Jun kinase 3 mitogen-activated protein kinase 10 stress activated protein kinase JNK3 stress activated protein kinase beta
Background Information	The stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation. The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.

Description

Catalogue Number

04-893

Replaces

05-893

Description

Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal

Alternate Names

JNK3 alpha protein kinase
MAP kinase
MAP kinase p49 3F12
Stress-activated protein kinase JNK3
c-Jun N-terminal kinase 3
c-Jun kinase 3
mitogen-activated protein kinase 10
stress activated protein kinase JNK3
stress activated protein kinase beta

Background Information

The stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation.
The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.

Product Information
Format	Culture Supernatant
Control	RIPA cell lysates from A431 cells.
Presentation	Cultured supernantant in 0.05% sodium azide.

Product Information

Format

Culture Supernatant

Control

RIPA cell lysates from A431 cells.

Presentation

Cultured supernantant in 0.05% sodium azide.

Applications
Application	Detect JNK3/SAPK1b using this Anti-JNK3/SAPK1b Antibody, clone C05T validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: A 1:500-1:2,000 dilution of this lot detected JNK3 in RIPA lysates from A431 cells.

Applications

Application

Detect JNK3/SAPK1b using this Anti-JNK3/SAPK1b Antibody, clone C05T validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: A 1:500-1:2,000 dilution of this lot detected JNK3 in RIPA lysates from A431 cells.

Biological Information
Immunogen	GST fusion protein corresponding to full-length rat JNK3/ SAPK1b
Clone	C05T
Host	Rabbit
Specificity	Recognizes JNK3/SAPK1b. Does not cross react with JNK1 or JNK2.
Isotype	IgG
Species Reactivity	Human Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002753.2
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
Gene Symbol	EC 2.7.11.24 FLJ12099 FLJ33785 JNK3 JNK3A MGC50974 OTTHUMP00000161180 OTTHUMP00000161182 OTTHUMP00000161183 PRKM10 p493F12 p54bSAPK
UniProt Number	P49187
UniProt Summary	FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity By similarity. Catalytic activity ATP + a protein = ADP + a phosphoprotein. Cofactor Magnesium By similarity. Enzyme regulation Activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K7 phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax for the enzyme. MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by HDAC9 By similarity. SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with HDAC9 and MAPKBP1 By similarity. DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM:Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme By similarity. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Molecular Weight	46-54kDa

Biological Information

Immunogen

GST fusion protein corresponding to full-length rat JNK3/ SAPK1b

Clone

C05T

Host

Rabbit

Specificity

Recognizes JNK3/SAPK1b. Does not cross react with JNK1 or JNK2.

Isotype

IgG

Species Reactivity

Human
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_002753.2

Entrez Gene Summary

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Gene Symbol

EC 2.7.11.24
FLJ12099
FLJ33785
JNK3
JNK3A
MGC50974
OTTHUMP00000161180
OTTHUMP00000161182
OTTHUMP00000161183
PRKM10
p493F12
p54bSAPK

UniProt Number

P49187

UniProt Summary

FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity By similarity.

Catalytic activity ATP + a protein = ADP + a phosphoprotein.

Cofactor Magnesium By similarity.

Enzyme regulation Activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K7 phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax for the enzyme. MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by HDAC9 By similarity.

SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with HDAC9 and MAPKBP1 By similarity.

DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

PTM:Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme By similarity.

SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Molecular Weight

46-54kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on RIPA lysates from A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on RIPA lysates from A431 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

a-JNK3/SAPK1b, clone C05T 100ul

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