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Anti-Pigment-Epithelium Derived Factor, clone 10F12.2 (mouse monoclonal)

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货号：MAB1059

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R ELISA, ICC, IHC, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB1059
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2
Alternate Names
  • pigment epithelium-derived factor
  • proliferation-inducing protein 35
  • serine (or cysteine) proteinase inhibitor
  • clade F (alpha-2
    antiplasmin, pigment epithelium derived factor), member 1
  • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin,pigment epithelium derived factor), member 1
Background Information Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001).
References
Product Information
Format Purified
Control
  • Eye tissue.
Presentation Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Applications
Application Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Western Blot:
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)

ELISA:
A previous lot of this antibody was used on ELISA.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.

Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.
Clone 10F12.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes Pigment-Epithelium Derived Factor (PEDF).
Isotype IgG2a
Species Reactivity
  • Human
  • Rat
Species Reactivity Note Human. Expected to react with rat.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SERPINF1
  • Serpin-F1
  • EPC-1
  • PEDF
  • PIG35
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
SIZE: 418 amino acids; 46342 Da
SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes.
TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma.
DEVELOPMENTAL STAGE: Expressed in quiescent cells.
DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the serpin family
Molecular Weight ~46 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on human placenta lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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