Ms X TNF-a

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, IHC, NEUT, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ICC, IHC, NEUT, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB1096
Replaces	04-1114
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Tumor Necrosis Factor-α Antibody, clone 195
Alternate Names	TNF-alpha

Description

Catalogue Number

MAB1096

Replaces

04-1114

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Tumor Necrosis Factor-α Antibody, clone 195

Alternate Names

TNF-alpha

Product Information
Format	Purified
Presentation	Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.

Product Information

Format

Purified

Presentation

Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.

Applications
Application	This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
Key Applications	Immunocytochemistry Immunohistochemistry Neutralizing Western Blotting
Application Notes	Western Blot Immunocytochemistry Immunohistochemistry Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D]. Optimal working dilutions must be determined by end user.

Applications

Application

This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.

Key Applications

Immunocytochemistry
Immunohistochemistry
Neutralizing
Western Blotting

Application Notes

Western Blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Human, recombinant tumor necrosis factor-alpha produced in E. coli.
Clone	195
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon. SPECIES REACTIVITIES: Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
Isotype	IgG3
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000594.2
Entrez Gene Summary	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Gene Symbol	TNF OTTHUMP00000037669 TNFA TNFSF2 cachectin TNF-a Cachectin DIF TNF-alpha
Non-Reactive Species	Canine Mouse Monkey Pig Rat Baboon
UniProt Number	P01375
UniProt Summary	FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. SIZE: 233 amino acids; 25644 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted. PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.

Biological Information

Immunogen

Human, recombinant tumor necrosis factor-alpha produced in E. coli.

Clone

195

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

SPECIES REACTIVITIES:

Not mouse, rat, canine, porcine, baboon or rhesus-monkey.

Isotype

IgG3

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000594.2

Entrez Gene Summary

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Gene Symbol

TNF
OTTHUMP00000037669
TNFA
TNFSF2
cachectin
TNF-a
Cachectin
DIF
TNF-alpha

Non-Reactive Species

Canine
Mouse
Monkey
Pig
Rat
Baboon

UniProt Number

P01375

UniProt Summary

FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
SIZE: 233 amino acids; 25644 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.

Storage and Shipping Information

Storage Conditions

Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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