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Anti-TRPC6 (Rb Polyclonal)

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货号：AB5574-200UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5574-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-TrpC6 Antibody
Alternate Names
  • Transient Receptor Potential-Like Channel 6
  • Trp6
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application This Anti-TrpC6 Antibody is validated for use in IH, WB for the detection of TrpC6.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1.5-3.0 μg/mL (1:200-1:400) using ECL on rat brain membranes.

Immunohistochemstry

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Peptide corresponding to amino acids 24-38 from mouse TRPC6 (Zhu et al. 1996). Accession Q61143
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes TRPC6 (Transient Receptor Potential-Like Channel 6, TRP-6). Does not cross react with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also work on human due to sequence homology. Other species have not been tested.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TRPC6
  • FLJ11098
  • FSGS2
  • TrpC6
  • FLJ14863
  • TRP6
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9Y210 # Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
SIZE: 931 amino acids; 106326 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
TISSUE SPECIFICITY: Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
DISEASE: SwissProt: Q9Y210 # Defects in TRPC6 are the cause of focal segmental glomerulosclerosis 2 (FSGS2) [MIM:603965]. FSGS2 is an autosomal dominant disease characterized by increased urinary protein excretion (proteinuria) and decreasing kidney function (nephrotic syndrome). Renal insufficiency often progresses to end-stage renal disease (ESRD) (also known as end-stage renal failure), a highly morbid state requiring either dialysis therapy or kidney transplantation.
SIMILARITY: SwissProt: Q9Y210 ## Belongs to the transient receptor family. STrpC subfamily. & Contains 4 ANK repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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