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Qiagen 凯杰 Biosharp Omega

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Anti-Dicer1, clone 5D12.2 (mouse monoclonal)

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号:04-721

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB M Purified Monoclonal Antibody
Description
Catalogue Number 04-721
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Dicer1 Antibody, clone 5D12.2
Alternate Names
  • DCR1
  • Dicer
  • HERNA
  • KIAA0928
Background Information RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).
References
Product Information
Format Purified
Applications
Application Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.
Key Applications
  • Western Blotting
Application Notes Western blot
Biological Information
Immunogen KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1
Clone 5D12.2
Host Mouse
Specificity Human Dicer1
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DICER1
  • DICER
  • HERNA
Purification Method Protein G Chromatography
UniProt Number
Molecular Weight 220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by western blot on lysates from HeLa nuclear extracts.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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