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Anti-Thy-1, clone M5/49 (rat monoclonal)

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货号：MABF34

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M WB, FUNC R Purified Monoclonal Antibody
Description
Catalogue Number MABF34
Description Anti-Thy-1 Antibody, clone M5/49
Alternate Names
  • Thy-1 cell surface antigen
  • Thy-1 T-cell antigen
  • Thy-1 membrane glycoprotein
  • Thy-1 antigen
  • CD90 antigen
Background Information Thy-1 membrane glycoprotein (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored surface protein belonging to the immunoglobulin superfamily and is a major cell surface protein found on both T–cells and in the brain. Thy-1 is thought to be involved in interactions taking place during synaptogenesis and other neurological events between cells or cells and their ligands. There are two known alleles for Thy-1; Thy1.1 and Thy1.2. Studies have shown N-glycosolation to take place at three locations on the protein.
References
Product Information
Format Purified
Control
  • P388D1 cell lysate
Presentation Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Thy-1 Antibody, clone M5/49 detects level of Thy-1 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
  • Affects Function
Application Notes Functional Assay: A previous lot of this antibody was used for the inhibition of B6 Anti-BNLΦ CTL mediated cell death. (Davignon, D., et al. (1981). PNAS USA. 78(7):4535-4539.)
Biological Information
Immunogen B6 Anti-BNL4Φ lymphocytes
Clone M549.4.1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Isotype IgG2aκ
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • THY1
  • CD90
  • Thy-1
  • CDw90
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor › GPI-anchor

POLYMORPHISM: There are two major alleles; Thy-1.1 (CD90.1) and Thy-1.2 (CD90.2).

SEQUENCE SIMILARITIES: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight ~22 kDa observed.
Higher bands may be observed due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in P388D1 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Thy-1 in 10 µg of P388D1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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