Anti-RAD51 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-1782
Replaces	MABE515
Description	Anti-RAD51 Antibody
Alternate Names	BRCA1/BRCA2-containing complex, subunit 5 DNA repair protein RAD51 homolog 1 RAD51 (S. cerevisiae) homolog (E coli RecA homolog) RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) RAD51 homolog protein RAD51 S.cerevisiae homolog of, A RecA-like protein recombination protein A
Background Information	RAD51 (previously known as DNA repair protein RAD51 homolog 1) is a member of the Rec A family involved in DNA repair. RAD51 interacts with BRCA1 and BRCA2, which are both involved with breast cancer, to activate homologous recombination and DNA damage repair. BRCA1 appears to be present in heredity forms of breast cancer that results in the transition of G to A on codon 150, thereby converting ARG to GLN. BRCA2 regulates intercellular localization and DNA binding ability. Loss of BRCA2 results in genomic instability and tumorgenesis. RAD51 acts as ATPase to repair double strand breaks and also unwinds DNA to form a helical nucleoprotein filament. RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.

Description

Catalogue Number

07-1782

Replaces

MABE515

Description

Anti-RAD51 Antibody

Alternate Names

BRCA1/BRCA2-containing complex, subunit 5
DNA repair protein RAD51 homolog 1
RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
RAD51 homolog protein
RAD51
S.cerevisiae
homolog of, A
RecA-like protein
recombination protein A

Background Information

RAD51 (previously known as DNA repair protein RAD51 homolog 1) is a member of the Rec A family involved in DNA repair. RAD51 interacts with BRCA1 and BRCA2, which are both involved with breast cancer, to activate homologous recombination and DNA damage repair. BRCA1 appears to be present in heredity forms of breast cancer that results in the transition of G to A on codon 150, thereby converting ARG to GLN. BRCA2 regulates intercellular localization and DNA binding ability. Loss of BRCA2 results in genomic instability and tumorgenesis. RAD51 acts as ATPase to repair double strand breaks and also unwinds DNA to form a helical nucleoprotein filament. RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Applications
Application	Use Anti-RAD51 Antibody (rabbit polyclonal antibody) validated in WB to detect RAD51 also known as BRCA1/BRCA2-containing complex subunit 5 ,DNA repair protein RAD51 homolog 1, RecA-like protein ,recombination protein A.
Key Applications	Western Blotting

Applications

Application

Use Anti-RAD51 Antibody (rabbit polyclonal antibody) validated in WB to detect RAD51 also known as BRCA1/BRCA2-containing complex subunit 5 ,DNA repair protein RAD51 homolog 1, RecA-like protein ,recombination protein A.

Key Applications

Western Blotting

Biological Information
Immunogen	Synthetic peptide corresponding to RAD51.
Host	Rabbit
Specificity	This antibody recognizes RAD51.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Tested on mouse. Predicted to react with human and rat based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_002866
Entrez Gene Summary	The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq].
Gene Symbol	BRCC5 HRAD51 HsRAD51 HsRad51 HsT16930 RAD51A RAD51D RAD51L3 RECA hRAD51
Purification Method	Affinity Purfied
UniProt Number	Q06609
UniProt Summary	Function: May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Ref.13 Subunit structure: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer By similarity. Interacts with OBFC2B. Subcellular location: Nucleus. Note= Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage. Ref.11 Ref.16 Tissue specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. Post-translational modification: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Ref.16 Involvement in disease: Defects in RAD51 are associated with breast cancer (BC) [MIM:114480]. Ref.20 Sequence similarities: Belongs to the recA family. RAD51 subfamily. Contains 1 HhH domain.
Molecular Weight	37 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to RAD51.

Host

Rabbit

Specificity

This antibody recognizes RAD51.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Tested on mouse. Predicted to react with human and rat based on sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_002866

Entrez Gene Summary

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq].

Gene Symbol

BRCC5
HRAD51
HsRAD51
HsRad51
HsT16930
RAD51A
RAD51D
RAD51L3
RECA
hRAD51

Purification Method

Affinity Purfied

UniProt Number

Q06609

UniProt Summary

Function: May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Ref.13
Subunit structure: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer By similarity. Interacts with OBFC2B.
Subcellular location: Nucleus. Note= Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage. Ref.11 Ref.16
Tissue specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Post-translational modification: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Ref.16
Involvement in disease: Defects in RAD51 are associated with breast cancer (BC) [MIM:114480]. Ref.20
Sequence similarities: Belongs to the recA family. RAD51 subfamily.
Contains 1 HhH domain.

Molecular Weight

37 kDa

Product Usage Statements
Quality Assurance	Western Blot Analysis: 1:500 dilution of this antibody was used to detect RAD51 on mouse testis lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis:
1:500 dilution of this antibody was used to detect RAD51 on mouse testis lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt.To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-RAD51 (rabbit polyclonal)

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