Anti-PDGF-BB (rabbit polyclonal)

Replacement Information
Replacement Information	07-1437 is a recommended replacement for AB1487P.

Replacement Information

07-1437 is a recommended replacement for AB1487P.

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ELISA, NEUT	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ELISA, NEUT

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-1437
Replaces	AB1487P
Description	Anti-PDGF-BB Antibody
Alternate Names	Platelet-derived growth factor B chain precursor Platelet-derived growth factor beta polypeptide Platelet-derived growth factor, beta polypeptide (oncogene SIS) oncogene SIS Platelet-derived growth factor Platelet-derived growth factor beta Platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) Platelet-derived growth factor, B chain V-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
Background Information	Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.

Description

Catalogue Number

07-1437

Replaces

AB1487P

Description

Anti-PDGF-BB Antibody

Alternate Names

Platelet-derived growth factor B chain precursor
Platelet-derived growth factor beta polypeptide
Platelet-derived growth factor, beta polypeptide (oncogene SIS)
oncogene SIS
Platelet-derived growth factor
Platelet-derived growth factor beta
Platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
Platelet-derived growth factor, B chain
V-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)

Background Information

Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.

Applications
Application	Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.
Key Applications	Western Blotting ELISA Neutralizing
Application Notes	ELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-BB is used to coat the plate. Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required. Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select® Detection with HRP-DAB. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.

Key Applications

Western Blotting
ELISA
Neutralizing

Application Notes

ELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of
0.2-0.4 ng/well when human PDGF-BB is used to coat the plate.

Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5
ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required.

Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select® Detection with HRP-DAB.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Highly purified (>98%) recombinant human PDGF-BB
Host	Rabbit
Specificity	Reacts with human PDGF-BB.
Isotype	IgG
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002608
Entrez Gene Summary	The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. [provided by RefSeq].
Gene Symbol	PDGFB FLJ12858 PDGF2 c-sis PDGF-2 SSV SIS Becaplermin
Purification Method	Affinity Purfied
UniProt Number	P01127
UniProt Summary	FUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 (By similarity). SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. DISEASE: A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities. PHARMACEUTICAL: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers. MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. SIMILARITY: Belongs to the PDGF/VEGF growth factor family.
Molecular Weight	28 kDa

Biological Information

Immunogen

Highly purified (>98%) recombinant human PDGF-BB

Host

Rabbit

Specificity

Reacts with human PDGF-BB.

Isotype

IgG

Species Reactivity

Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_002608

Entrez Gene Summary

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. [provided by RefSeq].

Gene Symbol

PDGFB
FLJ12858
PDGF2
c-sis
PDGF-2
SSV
SIS
Becaplermin

Purification Method

Affinity Purfied

UniProt Number

P01127

UniProt Summary

FUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 (By similarity).
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
DISEASE: A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
PHARMACEUTICAL: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.
MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
SIMILARITY: Belongs to the PDGF/VEGF growth factor family.

Molecular Weight

28 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PDGF-BB (rabbit polyclonal)

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