Anti-Src Family (WTAPE), clone 2E8.2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1461
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Src Family (WTAPE) Antibody, clone 2E8.2
Alternate Names	Integrin-linked kinase 59 kDa serine/threonine-protein kinase
Background Information	pp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain.

Description

Catalogue Number

05-1461

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Src Family (WTAPE) Antibody, clone 2E8.2

Alternate Names

Integrin-linked kinase
59 kDa serine/threonine-protein kinase

Background Information

pp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain.

Product Information
Format	Purified
Control	A431 Cell Lysate
Presentation	Protein G purified mouse monoclonal in PBS and 0.05% NaN3.

Product Information

Format

Purified

Control

A431 Cell Lysate

Presentation

Protein G purified mouse monoclonal in PBS and 0.05% NaN3.

Applications
Application	Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.
Key Applications	Western Blotting

Applications

Application

Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.

Key Applications

Western Blotting

Biological Information
Immunogen	Shared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE.
Epitope	C-term WTAPE region
Clone	2E8.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes Src Family Members at the following amino acids: Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428.
Isotype	IgG3κ
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human and Rat tested. Expected to react with Mouse.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_005408
Entrez Gene Summary	Summary for proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] (accession no. NP_005408) (one example of Src family proteins containing the immunogen sequence: other members include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk). The gene for the proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Gene Symbol	pp60c-src p60-Src c-Src
Purification Method	Protein G Purified
UniProt Number	P12931
UniProt Summary	Summary for SRC_HUMAN (accession no. P12931) (one example of Src family proteins containing the immunogen sequence: other members include Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk). CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 1 SH2 domain. SIMILARITY: Contains 1 SH3 domain.
Molecular Weight	~60 kDa

Biological Information

Immunogen

Shared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE.

Epitope

C-term WTAPE region

Clone

2E8.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes Src Family Members at the following amino acids:
Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428.

Isotype

IgG3κ

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human and Rat tested. Expected to react with Mouse.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_005408

Entrez Gene Summary

Summary for proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] (accession no. NP_005408) (one example of Src family proteins containing the immunogen sequence: other members include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).
The gene for the proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Gene Symbol

pp60c-src
p60-Src
c-Src

Purification Method

Protein G Purified

UniProt Number

P12931

UniProt Summary

Summary for SRC_HUMAN (accession no. P12931) (one example of Src family proteins containing the immunogen sequence: other members include Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 SH2 domain.
SIMILARITY: Contains 1 SH3 domain.

Molecular Weight

~60 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot Western Blot Analysis: This lot detected Src family members at a 2μg/mL dilution in EGF-treated A431 cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected Src family members at a 2μg/mL dilution in EGF-treated A431 cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Src Family (WTAPE), clone 2E8.2 (mouse monoclonal)

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