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Anti-IL-6, clone E457, Rabbit Monoclonal

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货号：04-1095

品牌：Millipore 密理博

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Replacement Information

特卖

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, IHC, IP, NEUT, WB Rb Serum Polyclonal Antibody
Description
Catalogue Number AB1431
Replaces 04-1095
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Interferon-β Antibody
Alternate Names
  • IFN-beta
Background Information Interferon beta is a globular protein consisting of 5 alpha helices with a predicted molecular weight of 20 kDa. Due to glycosylation, however, the observed molecular weight is closer to 25 kDa by SDS-PAGE.
References
Product Information
Format Serum
Control
  • IFN expressing cell lines
Presentation Rabbit Serum diluited with PBS. Liquid containing no preservatives.
Applications
Application This Anti-Interferon-β Antibody is validated for use in ELISA, IH, IP, NEUT, WB for the detection of Interferon-β.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Neutralizing
  • Western Blotting
Application Notes Interferon neutralization: 2 x 10e5 neutralization units/mL where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-b) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]; [Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for the effective neutralization of human interferon beta.

Immunohistochemistry

Western blot: 1:5000

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Recombinant human interferon beta expressed in E. coli
Host Rabbit
Specificity Recognizes Human interferon beta.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL6
  • HSF
  • BSF-2
  • HGF
  • IL-6
  • BSF2
  • CDF
  • IFNB2
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
SIZE: 212 amino acids; 23718 Da
SUBCELLULAR LOCATION: Secreted.
PTM: N- and O-glycosylated.
DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS.
SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily.
Molecular Weight 20 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 20000 units
Transport Information
Supplemental Information
Specifications

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