Anti-phospho-AS160 (Ser666) (rabbit polyclonal)

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Anti-phospho-AS160 (Ser666) (rabbit polyclonal)

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货号：09-489

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Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, B, Ca, Eq	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	09-489
Description	Anti-phospho-AS160 (Ser666) Antibody
Alternate Names	Acrg embryonic lethality minimal region ortholog Akt substrate of 160 kDa TBC (Tre-2, BUB2, CDC16) domain-containing protein TBC1 domain family, member 4
Background Information	AS160 (Akt Substrate of 160 kDa, TBC1D4) is a Rab-GAP protein that regulates the trafficking of GLUT4 (glucose transporter 4) to the plasma membrane. AS160 is known to be phosphorylated on at least 8 residues following stimulation from IGF-1, EGF, PMA, and AICAR (Chen, S., 2008). Thr642 is phosphorylated by Akt in response to IGF-1, while Ser341 and Thr642 of AS160 are phosphorylated by RSK (p90 ribosomal S6 kinase) in response to PMA. Ser588 has been shown to be phosphorylated by AMPK in vitro, though other kinases might be responsible as well. The phosphorylation site, Ser666, does not contain the Akt consensus sequence. Its phosphorylation is suppressed by AICAR and by combined mutations of TOS (mTOR signaling)-like sequence (FEMDI) and rapamycin (Geraghty, K.M., 2007). Additionally, its phosphorylation is markedly increased in response to IGF-1 and this phosphorylation is blocked in response to the PI3 Kinase inhibitor LY294002 (Geraghty, K.M., 2007).

References

Product Information
Format	Affinity Purified
Presentation	Affinity purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-phospho-AS160 (Ser666) Antibody is validated for use in WB for the detection of phospho-AS160 (Ser666).
Key Applications	Western Blotting

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids including and surrounding phosphorylated Ser666 of human AS160.
Epitope	Phosphorylated Ser666
Host	Rabbit
Specificity	This antibody detected AS160 when phosphorylated on Ser666.
Species Reactivity	Human Mouse Bovine Canine Horse
Species Reactivity Note	Human. Other species have not been tested, but immunogen shares 79% homology with mouse and 90% homology with cow, horse, and dog.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005535.1
Gene Symbol	AS160 DKFZp779C0666 KIAA0603 OTTHUMP00000018505 OTTHUMP00000040818
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	O60343
UniProt Summary	FUNCTION:May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. SUBCELLULAR LOCATION: Cytoplasm. Note= Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. TISSUE SPECIFICITY: Widely expressed, but differential expression for isoforms 1 and 2, with highest overall expression of isoform 2 in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Moderate isoform 2 expression in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. PTM: Phosphorylated by AKT1; insulin-induced. Ref.8 Ref.10 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. Involvement in disease May be involved in atopic dermatitis (AD). Ref.13 SEQUENCE SIMILARITY: Contains 2 PID domains. Contains 1 Rab-GAP TBC domain
Molecular Weight	160 kDa

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Product Usage Statements
Quality Assurance	Evaluated by western blot on untreated and IGF1 treated HEK293 cell lysate. Western Blot Analysis: 1:250 dilution of this antibody was used to detect AS160 phosphorylated on Ser666 in IGF1-treated HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt.

Packaging Information
Material Size	250 µL

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Anti-phospho-AS160 (Ser666) (rabbit polyclonal)

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