Anti-EHMT1 (C-terminus) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, ELISA, WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ICC, ELISA, WB

Purified

Polyclonal Antibody

Description
Catalogue Number	07-2045
Description	Anti-EHMT1 Antibody (CT)
Alternate Names	G9a like protein G9a-like protein 1 H3-K9-HMTase 5 Histone H3-K9 methyltransferase 5 Lysine N-methyltransferase 1D euchromatic histone methyltransferase 1 euchromatic histone-lysine N-methyltransferase 1
Background Information	EHMT1 (euchromatic histone methyltransferase 1), also known as G9a-like protein (GLP1), is a histone methyltransferase. This protein specifically methylates Lysine 9 of histone H3 in vitro. H3Lys9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is thought to be targeted to histone H3 by different DNA-binding proteins including E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.

Description

Catalogue Number

07-2045

Description

Anti-EHMT1 Antibody (CT)

Alternate Names

G9a like protein
G9a-like protein 1
H3-K9-HMTase 5
Histone H3-K9 methyltransferase 5
Lysine N-methyltransferase 1D
euchromatic histone methyltransferase 1
euchromatic histone-lysine N-methyltransferase 1

Background Information

EHMT1 (euchromatic histone methyltransferase 1), also known as G9a-like protein (GLP1), is a histone methyltransferase. This protein specifically methylates Lysine 9 of histone H3 in vitro. H3Lys9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is thought to be targeted to histone H3 by different DNA-binding proteins including E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.

Product Information
Format	Purified
Control	HeLa cells
Presentation	Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Product Information

Format

Purified

Control

HeLa cells

Presentation

Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Applications
Application	Anti-EHMT1 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of EHMT1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5 & has been validated in ICC, ELISA & WB.
Key Applications	Immunocytochemistry ELISA Western Blotting
Application Notes	Western Blot Analysis: This antibody is reported by an outside laboratory to be suitable for Western blotting using EHMT1-transfected 293 cells. ELISA: This antibody is reported by an outside laboratory to be suitable for ELISA.

Applications

Application

Anti-EHMT1 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of EHMT1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5 & has been validated in ICC, ELISA & WB.

Key Applications

Immunocytochemistry
ELISA
Western Blotting

Application Notes

Western Blot Analysis:
This antibody is reported by an outside laboratory to be suitable for Western blotting using EHMT1-transfected 293 cells.

ELISA:
This antibody is reported by an outside laboratory to be suitable for ELISA.

Biological Information
Immunogen	The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human EHMT1.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human
Species Reactivity Note	Recognizes human EMHT1. Not tested in other species.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_079033
Entrez Gene Summary	The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol	DEL9q34 DKFZp667M072 EC 2.1.1.43 EUHMTASE1 Eu-HMTase1 FLJ12879 FP13812 GLP GLP1 KIAA1876 KMT1D OTTHUMP00000022711 bA188C12.1
Purification Method	Protein A chromatography
UniProt Number	Q9H9B1
UniProt Summary	FUNCTION:Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. Catalytic activity S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.1 SUBUNIT STRUCTURE: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with WIZ and EHMT2. Ref.10 SUBCELLULAR LOCATION: Nucleus. Note= Associates with euchromatic regions. TISSUE SPECIFICITY: Widely expressed. Ref.5 DOMAIN:The SET domain mediates interaction with WIZ. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.11 DISEASE: Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Sequence similarities Belongs to the histone-lysine methyltransferase family. Contains 8 ANK repeats. Contains 1 pre-SET domain. Contains 1 SET domain. SEQUENCE CAUTION:The sequence CAD28534.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.
Molecular Weight	~138 kDa

Biological Information

Immunogen

The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human EHMT1.

Epitope

C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Species Reactivity

Human

Species Reactivity Note

Recognizes human EMHT1. Not tested in other species.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_079033

Entrez Gene Summary

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses
transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive
genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene Symbol

DEL9q34
DKFZp667M072
EC 2.1.1.43
EUHMTASE1
Eu-HMTase1
FLJ12879
FP13812
GLP
GLP1
KIAA1876
KMT1D
OTTHUMP00000022711
bA188C12.1

Purification Method

Protein A chromatography

UniProt Number

Q9H9B1

UniProt Summary

FUNCTION:Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle.

Catalytic activity S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.1

SUBUNIT STRUCTURE: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with WIZ and EHMT2. Ref.10

SUBCELLULAR LOCATION: Nucleus. Note= Associates with euchromatic regions.

TISSUE SPECIFICITY: Widely expressed. Ref.5

DOMAIN:The SET domain mediates interaction with WIZ.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.11

DISEASE: Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.

Sequence similarities Belongs to the histone-lysine methyltransferase family.

Contains 8 ANK repeats.

Contains 1 pre-SET domain.

Contains 1 SET domain.

SEQUENCE CAUTION:The sequence CAD28534.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Molecular Weight

~138 kDa

Product Usage Statements
Quality Assurance	Immunocytochemistry: The antibody detects EHMT1 in HeLa cell nuclei.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Immunocytochemistry:
The antibody detects EHMT1 in HeLa cell nuclei.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-EHMT1 (C-terminus) (rabbit polyclonal)

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