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Anti-PP6C (rabbit polyclonal)

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货号：07-1224

品牌：Millipore 密理博

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Replacement Information
Replacement Information 07-1224 is a recommended replacement for AB4091

特卖

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H, M, R WB, IP, IH(P) Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-1224
Replaces AB4091
Description Anti-PP6C Antibody
Alternate Names
  • H.sapiens mRNA for protein phosphatase 6
  • Serine/threonine-protein phosphatase 6
  • Protein phosphatase 6, catalytic subunit
  • Serine/threonine protein phosphatase catalytic subunit
Background Information This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.
References
Product Information
Format Purified
Control
  • Jurkat Cell Lysate
Presentation Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.
Applications
Application Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
Application Notes Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select®
detection with HRP-DAB.
Biological Information
Immunogen The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.
Isotype IgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. The N-terminal domain of this protein restricts G1 to S phase progression in cancer cells. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X.
Gene Symbol
  • PPP6C
  • PP6
  • PPP6
  • EC 3.1.3.16
UniProt Number
UniProt Summary FUNCTION: SwissProt: O00743 # May function in cell cycle regulation.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 305 amino acids; 35144 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Highest expression levels in testis, heart, and skeletal muscle and lowest in placenta, lung and kidney.
SIMILARITY: SwissProt: O00743 ## Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
Molecular Weight Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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