Anti-REST (rabbit polyclonal)

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Anti-REST (rabbit polyclonal)

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货号：09-019

品牌：Millipore 密理博

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北京朝阳

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	09-019
Description	Anti-REST Antibody
Alternate Names	Neural-restrictive silencer factor RE1-silencing transcription factor X2 box repressor neuron restrictive silencer factor repressor binding to the X2 box
Background Information	REST (RE1-silencing transcription factor) also known as NRSF (neuron-restrictive silencer factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of REST to the NRSE represses neuronal gene transcription in non-neuronal cells. Although REST is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. REST contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. REST associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for REST outside the nervous system. Down-regulation of REST, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. REST is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

References

Product Information
Format	Affinity Purified
Control	HeLa nuclear extract
Presentation	Affinity purified rabbit serum in PBS with 0.07% sodium azide, and 30% glycerol.

Applications
Application	This Anti-REST Antibody is validated for use in WB for the detection of REST.
Key Applications	Western Blotting

Biological Information
Immunogen	GST fusion protein corresponding to residues 801-1097 of human REST.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes REST
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_005612
Entrez Gene Summary	This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined. [provided by RefSeq]
Gene Symbol	NRSF XBR
Purification Method	Antigen Affinity Purified
UniProt Number	Q13127
UniProt Summary	Function: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Subunit structure: Interacts with SIN3A, SIN3B and RCOR1. Subcellular location: NucleusProbable. Tissue specificity: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. Sequence similarities: Contains 9 C2H2-type zinc fingers. Sequence caution: The sequence AAA98503.1 differs from that shown. Reason: Frameshift at positions 189, 212 and 1087. The sequence AAH38985.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.
Molecular Weight	120 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by western blot with HeLa nuclear extract. Western Blot Analysis: 0.5-1 μg/mL of this antibody detected REST in nuclear extracts from HeLa, Raji, and Jurkat cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.