Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
Vrt	ELISA, IHC, ICC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Vrt

ELISA, IHC, ICC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1313
Description	Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
Alternate Names	ubiquitin C polyubiquitin B ubiquitin B
Background Information	Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Description

Catalogue Number

05-1313

Description

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4

Alternate Names

ubiquitin C
polyubiquitin B
ubiquitin B

Background Information

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Product Information
Format	Purified
Control	Lys63 polyubiquitin chains.
Presentation	Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Product Information

Format

Purified

Control

Lys63 polyubiquitin chains.

Presentation

Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Applications
Application	Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
Key Applications	ELISA Immunohistochemistry Immunocytochemistry Western Blotting
Application Notes	Western Blot Analysis: A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting. Additional Research Applications Immunocytochemistry: This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin. Immunohistochemistry: This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer's patient. ELISA: This antibody has been reported by an outside laboratory to be suitable for ELISA.

Applications

Application

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.

Key Applications

ELISA
Immunohistochemistry
Immunocytochemistry
Western Blotting

Application Notes

Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications

Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.

Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer's patient.

ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.

Biological Information
Immunogen	Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).
Epitope	Lys63 ubiquitination site
Clone	HWA4C4
Host	Mouse
Specificity	Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Isotype	IgG2a
Species Reactivity	Vertebrates
Species Reactivity Note	Human. Based on sequence homology, broad species cross-reactivity is expected.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_018955.2
Entrez Gene Summary	This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]
Gene Symbol	UBB UBC FLJ25987 MGC8385 OTTHUMP00000064960 OTTHUMP00000064961 UBA80 UBCEP1 UBCEP2
UniProt Number	P62988
UniProt Summary	FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly. MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a). SIMILARITY: Belongs to the ubiquitin family.
Molecular Weight	varies

Biological Information

Immunogen

Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).

Epitope

Lys63 ubiquitination site

Clone

HWA4C4

Host

Mouse

Specificity

Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Isotype

IgG2a

Species Reactivity

Vertebrates

Species Reactivity Note

Human. Based on sequence homology, broad species cross-reactivity is expected.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_018955.2

Entrez Gene Summary

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]

Gene Symbol

UBB
UBC
FLJ25987
MGC8385
OTTHUMP00000064960
OTTHUMP00000064961
UBA80
UBCEP1
UBCEP2

UniProt Number

P62988

UniProt Summary

FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIMILARITY: Belongs to the ubiquitin family.

Molecular Weight

varies

Product Usage Statements
Quality Assurance	Western Blot Analysis: A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 (mouse monoclonal)

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