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Anti-Unc84B/SUN2 (rabbit polyclonal)

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货号：06-1038

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Ca, Eq IP, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 06-1038
Description Anti-Unc84B/SUN2 Antibody
Alternate Names
  • Rab5-interacting protein
  • Sad1 unc-84 domain protein 2
  • Sad1/unc-84 protein-like 2
  • nuclear envelope protein
  • unc-84 homolog B
  • unc-84 homolog B (C. elegans)
Background Information UNC84B (Protein UNC-84 homolog B or SUN2), is a single-pass nuclear envelope transmembrane protein responsible for nuclear migrations that are essential for development. UNC-84B has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. UNC-84B interacts with RAB5A and is widely expressed, and highly expressed in the heart, lungs and muscles. UNC-84 may function to facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage.
References
Product Information
Format Affinity Purified
Control
  • U2OS cell lysate.
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application This Anti-Unc84B/SUN2 Antibody is validated for use in Immunoprecipitation and Western Blotting for the detection of Unc84B/SUN2.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Unc84B/SUN2 from 500 µg of U2OS cell lysate.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human Unc84B/SUN2 at and around the N-terminus.
Epitope N-terminus
Concentration 0.5 mg/mL
Host Rabbit
Specificity This antibody recognizes Unc84B/SUN2 at the N-terminus.
Isotype IgG
Species Reactivity
  • Human
  • Canine
  • Horse
Species Reactivity Note Proven to react with human. Predicted to react with dog and horse based on sequence homology. Mouse and rat (87%).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FRIGG
  • UNC84B
  • RAB5IP
  • Rab5IP
  • SUN2
Purification Method Affinity Purfied
UniProt Number
UniProt Summary SUBUNIT STRUCTURE: Interacts with RAB5A.

SUBCELLULAR LOCATION: Nucleus membrane; Single-pass membrane protein. Endosome membrane; Single-pass membrane protein Probable.

TISSUE SPECIFICTY: Widely expressed. Highly expressed in heart, lung and muscle. Weakly expressed in fetal heart. Slightly overexpressed in some heart tissues form patients with congenital heart defects.

DOMAIN: The Unc84 domain may play a role in the nuclear anchoring and/or migration.

SEQUENCE SIMILARITIES: Contains 1 Unc84 (SUN) domain.

Caution It is uncertain whether Met-1 or Met-50 is the initiator.
Molecular Weight ~80 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in U2OS cell lysate.

Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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