Anti-FOXO4 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-1720
Replaces	MABE539; MABD181
Description	Anti-FOXO4 Antibody
Alternate Names	Fork head domain transcription factor AFX1 forkhead box O4 myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
Background Information	Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response.

Description

Catalogue Number

07-1720

Replaces

MABE539; MABD181

Description

Anti-FOXO4 Antibody

Alternate Names

Fork head domain transcription factor AFX1
forkhead box O4
myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)

Background Information

Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response.

Product Information
Format	Affinity Purified
Control	Mouse kidney lysate.
Presentation	Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Product Information

Format

Affinity Purified

Control

Mouse kidney lysate.

Presentation

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Applications
Application	Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry: A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.

Applications

Application

Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.

Biological Information
Immunogen	Synthetic peptide corresponding to the N-terminal region of FOXO4.
Epitope	N-terminus
Host	Rabbit
Specificity	This antibody recognizes FOXO4 at the N-terminus.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Tested on human and mouse. Predicted to react with rat based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005929
Gene Symbol	AFX AFX1 MGC120490 MLLT7 OTTHUMP00000023496
Purification Method	Affinity Purfied
UniProt Number	P98177
UniProt Summary	FUNCTION: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 SUBUNIT: Interacts with CBP, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Ref.10 Ref.12 Ref.11 SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Ref.11 Ref.7 TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. PTM: Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. DISEASE: A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. Pharmaceutical use A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity. SIMILARITY: Contains 1 fork-head DNA-binding domain.
Molecular Weight	~65 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to the N-terminal region of FOXO4.

Epitope

N-terminus

Host

Rabbit

Specificity

This antibody recognizes FOXO4 at the N-terminus.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Tested on human and mouse. Predicted to react with rat based on sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_005929

Gene Symbol

AFX
AFX1
MGC120490
MLLT7
OTTHUMP00000023496

Purification Method

Affinity Purfied

UniProt Number

P98177

UniProt Summary

FUNCTION: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Ref.6 Ref.8 Ref.9 Ref.10 Ref.12

SUBUNIT: Interacts with CBP, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Ref.10 Ref.12 Ref.11

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Ref.11 Ref.7

TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

PTM: Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.

Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.

DISEASE: A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.

Pharmaceutical use A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity.

SIMILARITY: Contains 1 fork-head DNA-binding domain.

Molecular Weight

~65 kDa

Product Usage Statements
Quality Assurance	Western Blot Analysis: 1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt. To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-FOXO4 (rabbit polyclonal)

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