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Anti-FXR1, clone 6BG10 (mouse monoclonal)

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货号：05-1529

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, IP M Purified Monoclonal Antibody
Description
Catalogue Number 05-1529
Description Anti-FXR1 Antibody, clone 6BG10
Alternate Names
  • fragile X mental retardation, autosomal homolog 1
  • fragile X mental retardation-related protein 1
Background Information Fragile X mental retardation-related protein 1 (FXR1) and FXR2 are members of the FMR1 family sharing a very high degree of protein similarity among them. They interact with each other and also with FMR1, moving between the cytoplasm and the nucleus. FXR1 is an RNA binding protein and is expressed in the heart, kidney, brain, and testis. Mutations in FMR1 result in the most common form of mental retardation.
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application Use Anti-FXR1 Antibody, clone 6BG10 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect FXR1 also known as fragile X mental retardation autosomal homolog 1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application Notes Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FXR1 in HeLa and NIH/3T3 cell lysates.

Immunoprecipitation Analysis: A representative lot was used in an independent laboratory in immunoprecipitation. Siomi, M., et al. (1996). Molecular and Cellular Biology. 3825-3832.
Biological Information
Immunogen Recombinant protein corresponding to human FXR1.
Epitope Unknown
Clone 6BG10
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes FXR1.
Isotype IgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol
  • FXR1
  • hFXR1
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs By similarity.

SUBUNIT STRUCTURE: Interacts with FMR1 and FXR2. Interacts with TDRD3, and with CYFIP2 but not with CYFIP1.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICTY: Expressed in all tissues examined including heart, brain, kidney and testis.

PTM: Arg-445 is dimethylated, probably to asymmetric dimethylarginine.

SEQUENCE SIMILARITIES: Belongs to the FMR1 family.

Contains 2 KH domains.
Molecular Weight 68 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected FXR1 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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