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Anti-Puma BH3 domain

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号:AB10418

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB10418
Description Anti-Puma BH3 domain Antibody
Alternate Names
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • p53 up-regulated modulator of apoptosis
Background Information PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis
References
Product Information
Format Purified
Control
  • Western Blot : Mouse brain lysate
Presentation Purified in PBS with 0.09% NaN3.
Applications
Application Anti-Puma BH3 domain Antibody is an antibody against Puma BH3 domain for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen This antibody was developed against a synthetic peptide selected from the C-terminal region of human Puma.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity The antibody reacts with the 21 kDa Puma BH3 domain protein.
Species Reactivity
  • Mouse
Species Reactivity Note Reacts with mouse. Other species have not been tested.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • JFY-1
  • JFY1
  • PUMA
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Essential mediator of p53-dependent and p53-independent apoptosis.
SUBUNIT STRUCTURE: Interacts with MCL1 and BCL2A1 By similarity. Interacts with BCL2 and BCL2L1/BCL-XL.
SUBCELLULAR LOCATION: Mitochondrion. Note= Localized to the mitochondria in order to induce cytochrome c release.
TISSUE SPECIFICTY: Ubiquitously expressed.
INDUCTION: By DNA damage, glucocorticoid treatment, growth factor deprivation and p53.
SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.
Molecular Weight 21 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Western Blot Analysis: 1:250 dilution of this antibody detected Puma BH3 domain on mouse brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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