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Anti-LIMK1, clone 40/00-1B11-1-1 (rat monoclonal)

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货号：MAB10750

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP, ICC, IHC R Purified Monoclonal Antibody
Description
Catalogue Number MAB10750
Description Anti-LIMK1 Antibody, clone 40/00-1B11-1-1
Alternate Names
  • LIM domain kinase 1
  • LIM motif-containing protein kinase
Background Information LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.
References
Product Information
Format Purified
Control
  • PC3 cell lysate
Presentation Purified in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application This Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 is validated for use in WB, IP, IC, IH for the detection of LIMK1.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Biological Information
Immunogen GST-tagged recombinant protein corresponding to a region of mouse LIMK1.
Epitope Unknown
Clone 40/00-1B11-1-1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Specificity The antibody recognizes LIMK1.
Isotype IgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Human. Predicted to react with mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
Gene Symbol
  • Limk
  • Limk1
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

SUBUNIT STURCTURE: Self-associates. Binds ROCK1 By similarity. The LIM domain interacts with the cytoplasmic domain of NRG1. Interacts with NISCH and SSH1.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Highest expression in the nervous system, particularly in the spinal cord and the cranial nerve and dorsal root ganglia.

PTM: Autophosphorylated By similarity.

Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.

INVOLVEMENT IN DISEASE: Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 2 LIM zinc-binding domains.

Contains 1 PDZ (DHR) domain.

Contains 1 protein kinase domain.
Molecular Weight ~ 73 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected LIMK1 on 10 µg of PC3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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