Anti-Rac1b (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IH(P), IF	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, IH(P), IF

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-271
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Rac1b Antibody
Alternate Names	Ras-related C3 botulinum toxin substrate 1 Cell migration-inducing gene 5 protein Ras-like protein TC25 p21-Rac1
Background Information	Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Description

Catalogue Number

09-271

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Rac1b Antibody

Alternate Names

Ras-related C3 botulinum toxin substrate 1
Cell migration-inducing gene 5 protein
Ras-like protein TC25
p21-Rac1

Background Information

Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.

Applications
Application	Anti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Immunofluorescence
Application Notes	Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.

Applications

Application

Anti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence

Application Notes

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_061485.1
Entrez Gene Summary	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq] Transcript Variant: This variant (Rac1b) includes the alternatively spliced 57 bp region (exon 3b) that is missing in transcript variant Rac1.
Gene Symbol	RAC1 MGC111543 ORFNames=MIG5 PAK1 MIG5 TC-25 p21-Rac1
Purification Method	Affinity Purfied
UniProt Number	P63000
UniProt Summary	FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction. SIZE: 192 amino acids; 21450 Da SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6. SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
Molecular Weight	~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.

Species Reactivity

Human
Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_061485.1

Entrez Gene Summary

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
Transcript Variant: This variant (Rac1b) includes the alternatively spliced 57 bp region (exon 3b) that is missing in transcript variant Rac1.

Gene Symbol

RAC1
MGC111543
ORFNames=MIG5
PAK1
MIG5
TC-25
p21-Rac1

Purification Method

Affinity Purfied

UniProt Number

P63000

UniProt Summary

FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
SIZE: 192 amino acids; 21450 Da
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
SIMILARITY: Belongs to the small GTPase superfamily. Rho family.

Molecular Weight

~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MCF7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Rac1b (rabbit polyclonal)

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