Anti-Akt2/PKBb, clone AW114 (rabbit monoclonal) - 炼石商城

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Anti-Akt2/PKBb, clone AW114 (rabbit monoclonal)

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货号：04-771

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IP	Rb	Purified	Monoclonal Antibody

Description
Catalogue Number	04-771
Replaces	04-406
Description	Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal
Alternate Names	AKT2 kinase Murine thymoma viral (v-akt) homolog-2 PKB beta Protein kinase Akt-2 Protein kinase B, beta rac protein kinase beta v-akt murine thymoma viral oncogene homolog 2
Background Information	Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).

References

Product Information
Format	Purified
Control	L6 cell lysate
Presentation	Cultured supernantant containing 0.05% sodium azide.

Applications
Application	Anti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation

Biological Information
Immunogen	KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.
Epitope	a.a. 455-469
Clone	AW114
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Akt2/PKBβ, Mr ~60 kDa.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human and rat. Predicted to cross-react with mouse.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001617.1
Entrez Gene Summary	This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Gene Symbol	EC 2.7.11.1 PKBB PKBBETA PRKBB RAC-BETA RAC-PK-beta
UniProt Number	P31751
UniProt Summary	FUNCTION: General protein kinase capable of phosphorylating several known proteins. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation. SUBUNIT STRUCTURE: Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. TISSUE SPECIFICITY:In all human cell types so far analyzed. INVOLVEMENT IN DISEASE: Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain.
Molecular Weight	~60 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Western Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µl

Transport Information

Supplemental Information

Specifications

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